缺氧对 HL-1 心肌细胞鸢尾素表达的影响。
The Effect of Hypoxia on Irisin Expression in HL-1 Cardiomyocytes.
机构信息
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland;
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
出版信息
In Vivo. 2024 Sep-Oct;38(5):2126-2133. doi: 10.21873/invivo.13675.
BACKGROUND/AIM: Cardiovascular diseases (CVD) are the leading cause of death worldwide. In 2019, 523 million people were diagnosed with CVD, with 18.6 million deaths. Improved treatment and diagnostics could reduce CVD's impact. Irisin (Ir) is crucial for heart function and may be a biomarker for heart attack. Ir is a glycoprotein with sugar residues attached to its protein structure. This glycosylation affects Ir stability, solubility, and receptor interactions on target cells. Its secondary structure includes a fibronectin type III domain, essential for its biological functions. Ir helps cardiomyocytes to respond to hypoxia and protects mitochondria. The aim of the study was to determine the FNDC5 gene expression level and the Ir level in HL-1 cardiomyocytes subjected to hypoxia.
MATERIALS AND METHODS
We examined the effect of hypoxia on the expression levels of the FNDC5 gene and those of Ir in mouse cardiomyocytes of the HL-1 cell line. Real-time PCR (RT-PCR) was used to estimate the expression levels of the FNDC5 gene. Western blot and immunofluorescence methods were used to analyze the Ir protein levels.
RESULTS
Analyses showed an increased Ir level in HL-1 cardiomyocytes in response to hypoxia. This is the first study to confirm the presence of Ir in HL-1 cells.
CONCLUSION
The observed increase in Ir expression in murine cardiomyocytes is associated with the hypoxic environment and can be potentially used to diagnose hypoxia and CVD.
背景/目的：心血管疾病（CVD）是全球范围内的主要死因。2019 年，全球有 5.23 亿人被诊断患有 CVD，其中 1860 万人死亡。改善治疗和诊断方法可以降低 CVD 的影响。鸢尾素（Ir）对心脏功能至关重要，可能是心脏病发作的生物标志物。Ir 是一种糖蛋白，其蛋白质结构上附着有糖基。这种糖基化会影响 Ir 的稳定性、溶解度和靶细胞上的受体相互作用。其二级结构包括一个纤维连接蛋白 III 结构域，这对其生物学功能至关重要。Ir 帮助心肌细胞对缺氧做出反应并保护线粒体。本研究旨在确定 HL-1 心肌细胞在缺氧条件下 FNDC5 基因表达水平和 Ir 水平。
材料和方法
我们研究了缺氧对 HL-1 心肌细胞中 FNDC5 基因表达水平和 Ir 水平的影响。实时 PCR（RT-PCR）用于估计 FNDC5 基因的表达水平。Western blot 和免疫荧光法用于分析 Ir 蛋白水平。
结果
分析表明，HL-1 心肌细胞在缺氧时 Ir 水平升高。这是首次在 HL-1 细胞中证实存在 Ir。
结论
观察到在鼠心肌细胞中 Ir 表达增加与缺氧环境有关，可用于诊断缺氧和 CVD。
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