Glycoscience Group, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Methods Mol Biol. 2022;2460:223-237. doi: 10.1007/978-1-0716-2148-6_14.
The use of glycan microarrays to study carbohydrate interactions of bacterial cells is of great interest owing to the key roles these interactions play in bacterial colonization and infection of a host. In this chapter, the methods to fluorescently stain Gram-positive or Gram-negative bacteria and profiling them for glycan interactions using glycan microarrays are described in detail. The application of the Student's t-test to glycan microarray data using an example data set comparing glycan microarray binding of an Acinetobacter baumannii wild type and mutant strain is also described in step-by-step detail.
糖基微阵列在研究细菌细胞中碳水化合物相互作用方面具有重要意义，因为这些相互作用在细菌定植和宿主感染中起着关键作用。本章详细描述了使用糖基微阵列对革兰氏阳性或革兰氏阴性细菌进行荧光染色并对其糖基相互作用进行分析的方法。还详细介绍了如何使用学生 t 检验对糖基微阵列数据进行分析，并使用比较鲍曼不动杆菌野生型和突变株糖基微阵列结合的示例数据集一步一步地说明。
