Carbohydrate Signalling Group, Discipline of Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Methods Mol Biol. 2022;2460:239-248. doi: 10.1007/978-1-0716-2148-6_15.
Mammalian cell surface lectins mediate many important biological interactions which regulate physiological processes and therefore profiling mammalian cells on glycan microarray is of interest. However, many whole mammalian cells are not compatible with glycomics microarray formats and instead cell-derived micelles are prepared and profiled instead of whole cells as they can accurately represent the parental cell glycome. In this chapter, we describe the preparation of cell-derived micelles from mammalian cells, their labeling using a membrane-incorporating dye, and their profiling on a glycan microarray platform.
哺乳动物细胞表面的凝集素介导许多重要的生物学相互作用，这些相互作用调节生理过程，因此对糖组学微阵列上的哺乳动物细胞进行分析是很有意义的。然而，许多完整的哺乳动物细胞与糖组学微阵列格式不兼容，因此制备并分析细胞衍生的胶束而不是整个细胞，因为它们可以准确地代表亲代细胞的聚糖组。在本章中，我们描述了从哺乳动物细胞制备细胞衍生胶束的方法，使用一种膜整合染料对其进行标记，并在糖组学微阵列平台上对其进行分析。
