Carbohydrate Signalling Group, Discipline of Microbiology, National University of Ireland Galway, H91 TK33 Galway, Ireland.
Infectious Disease Laboratory, Discipline of Microbiology, National University of Ireland Galway, H91 TK33 Galway, Ireland.
Int J Mol Sci. 2020 Apr 2;21(7):2465. doi: 10.3390/ijms21072465.
The biofilm component poly--acetylglucosamine (PNAG) is an important virulence determinant in medical-device-related infections caused by ESKAPE group pathogens including Gram-positive and Gram-negative . PNAG presentation on bacterial cell surfaces and its accessibility for host interactions are not fully understood. We employed a lectin microarray to examine PNAG surface presentation and interactions on methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) and a clinical isolate. Purified PNAG bound to wheatgerm agglutinin (WGA) and succinylated WGA (sWGA) lectins only. PNAG was the main accessible surface component on MSSA but was relatively inaccessible on the surface, where it modulated the presentation of other surface molecules. Carbohydrate microarrays demonstrated similar specificities of and for their most intensely binding carbohydrates, including 3' and 6'sialyllactose, but differences in moderately binding ligands, including blood groups A and B. An -acetylglucosamine-binding lectin function which binds to PNAG identified on the cell surface may contribute to biofilm structure and PNAG surface presentation on . Overall, these data indicated differences in PNAG presentation and accessibility for interactions on Gram-positive and Gram-negative cell surfaces which may play an important role in biofilm-mediated pathogenesis.
生物膜成分聚乙酰葡萄糖胺（PNAG）是由 ESKAPE 组病原体引起的与医疗器械相关感染的重要毒力决定因素，包括革兰氏阳性菌和革兰氏阴性菌。PNAG 在细菌表面的呈现及其与宿主相互作用的可及性尚未完全了解。我们使用凝集素微阵列来检测耐甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）以及临床分离株的 PNAG 表面呈现和相互作用。纯化的 PNAG 仅与麦胚凝集素（WGA）和琥珀酰化 WGA（sWGA）凝集素结合。PNAG 是 MSSA 上主要的可及表面成分，但在 表面上相对不可及，在那里它调节其他表面分子的呈现。碳水化合物微阵列显示 与 对其结合最强烈的碳水化合物（包括 3'和 6'唾液乳糖）具有相似的特异性，但在中度结合配体（包括血型 A 和 B）上存在差异。一种可能存在于 细胞表面上的结合 PNAG 的 N-乙酰葡萄糖胺结合凝集素功能可能有助于生物膜结构和 PNAG 在 表面的呈现。总的来说，这些数据表明革兰氏阳性菌和革兰氏阴性菌表面上 PNAG 呈现和相互作用的可及性存在差异，这可能在生物膜介导的发病机制中发挥重要作用。
