聚糖微阵列的制备与分析
Preparation and analysis of glycan microarrays.
作者信息
Heimburg-Molinaro Jamie, Song Xuezheng, Smith David F, Cummings Richard D
机构信息
The Glycomics Center at Emory University School of Medicine, Atlanta, Georgia, USA.
出版信息
Curr Protoc Protein Sci. 2011 Apr;Chapter 12:Unit12.10. doi: 10.1002/0471140864.ps1210s64.
Abstract
Determination of the binding specificity of glycan-binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious. The advent of glycan microarrays has revolutionized the field of glycobiology by allowing simultaneous screening of a GBP for interactions with a large set of glycans in a single format. This unit describes the theory and method for production of two types of glycan microarrays (chemo/enzymatically synthesized and naturally derived), and their application to functional glycomics to explore glycan recognition by GBPs. These procedures are amenable to various types of arrays and a wide range of GBP samples.
摘要
传统上，确定聚糖结合蛋白（GBP）（如凝集素、抗体和受体）的结合特异性既困难又费力。聚糖微阵列的出现彻底改变了糖生物学领域，它允许以单一形式同时筛选GBP与大量聚糖的相互作用。本单元描述了两种类型聚糖微阵列（化学/酶促合成和天然来源）的制备理论和方法，以及它们在功能糖组学中的应用，以探索GBP对聚糖的识别。这些程序适用于各种类型的阵列和广泛的GBP样品。
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