Li Zongjie, Li Yuhao, Xiao Changguang, Yan Zujie, Pan Ruyi, Gao Yun, Li Beibei, Wei Jianchao, Qiu Yafeng, Liu Ke, Shao Donghua, Ma Zhiyong
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Microbiol Res. 2022 Mar;256:126954. doi: 10.1016/j.micres.2021.126954. Epub 2021 Dec 24.
Lactic acid bacteria that inhabit in the lung play important roles in maintaining the microbiome balance by interacting with the host immune system. Numerous metabolites (e.g., short chain fatty acids, bacteriocins, and hydrogen peroxide) produced by Lactobacillus sakei possess a special inhibitory spectrum against invading pathogens. In this research, the whole genome of L. sakei JD10 strain isolated from the porcine lung was sequenced and investigated. The whole size of the L. sakei JD10 chromosome was 1,989,921 bp, which encoded a total of 1951 predicted genes. Genome analyses revealed that many genes encoded carbohydrate-active enzymes (CAZymes) were predicted, which were responsible for the carbohydrate degradation and short chain fatty acids production. The metabolic profiles of short chain fatty acids in the L. sakei JD10 culture medium were measured by GC/TOFMS, and their regulatory effects on bacterial phagocytosis of RAW264.7 cells were also determined. The bacteriocin-producing genes of the L. sakei JD10 genome were also predicted, and a bacteriocin gene encoding carnocin was characterized and its molecular structure was analyzed. Two CRISPR-Cas system related genes were identified from the L. sakei JD10 genome, revealed that precise and efficient genome editing technologies could be applied for genetic engineering-manipulation. In all, investigation on the genomic features and metabolic features of L. sakei JD10 showed the potential probiotic traits to fight against pathogenic infection and regulate the host immune function.
栖息于肺部的乳酸菌通过与宿主免疫系统相互作用，在维持微生物群平衡方面发挥着重要作用。清酒乳杆菌产生的多种代谢产物（如短链脂肪酸、细菌素和过氧化氢）对入侵病原体具有特殊的抑制谱。在本研究中，对从猪肺中分离出的清酒乳杆菌JD10菌株的全基因组进行了测序和研究。清酒乳杆菌JD10染色体的全长为1,989,921 bp，共编码1951个预测基因。基因组分析显示，预测出许多编码碳水化合物活性酶（CAZymes）的基因，这些基因负责碳水化合物降解和短链脂肪酸的产生。通过GC/TOFMS测定了清酒乳杆菌JD10培养基中短链脂肪酸的代谢谱，并确定了它们对RAW264.7细胞细菌吞噬作用的调节作用。还预测了清酒乳杆菌JD10基因组中产生细菌素的基因，对一个编码肉毒杆菌素的细菌素基因进行了表征并分析了其分子结构。从清酒乳杆菌JD10基因组中鉴定出两个与CRISPR-Cas系统相关的基因，表明精确高效的基因组编辑技术可应用于基因工程操作。总之，对清酒乳杆菌JD10的基因组特征和代谢特征的研究表明，其具有对抗病原体感染和调节宿主免疫功能的潜在益生菌特性。
