College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China; College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, China.
Ultrason Sonochem. 2022 Jan;82:105901. doi: 10.1016/j.ultsonch.2021.105901. Epub 2021 Dec 28.
The controllable ultrasonic modification was hindered due to the uncertainty of the relationship between ultrasonic parameters and polysaccharide quality. In this study, the ultrasonic degradation process was established with kinetics. The physicochemical properties and prebiotic activity of ultrasonic degraded Flammulina velutipes polysaccharides (U-FVPs) were investigated. The results showed that the ultrasonic degradation kinetic models were fitted to 1/M-1/M = kt. When the ultrasonic intensity increased from 531 to 3185 W/cm, the degradation proceeded faster. The decrease of polysaccharide concentration contributed to the degradation of FVP, and the fastest degradation rate was at 60 °C. Ultrasound changed the solution conformation of FVP, and partially destroyed the stability of the triple helix structure of FVP. Additionally, the viscosity and gel strength of FVP decreased, but its thermal stability was improved by ultrasound. Higher ultrasonic intensity led to larger variations in physicochemical properties. Compared with FVP, U-FVPs could be more easily utilized by gut microbiota. U-FVPs displayed better prebiotic activity by promoting the growth of Bifidobacterium and Brautella and inhibiting the growth of harmful bacteria. Ultrasound could be effectively applied to the degradation of FVP to improve its physicochemical properties and bioactivities.
由于超声参数与多糖质量之间关系的不确定性，可控超声修饰受到阻碍。在本研究中，通过动力学建立了超声降解过程。研究了超声降解金针菇多糖（U-FVPs）的理化性质和益生元活性。结果表明，超声降解动力学模型拟合为 1/M-1/M=kt。当超声强度从 531 增加到 3185 W/cm 时，降解速度加快。多糖浓度的降低有助于 FVP 的降解，最快的降解速率出现在 60°C。超声改变了 FVP 的溶液构象，并部分破坏了 FVP 三螺旋结构的稳定性。此外，FVP 的粘度和凝胶强度降低，但超声使其热稳定性提高。更高的超声强度导致理化性质的变化更大。与 FVP 相比，U-FVPs 可以更被肠道微生物群利用。U-FVPs 通过促进双歧杆菌和布拉氏菌的生长和抑制有害细菌的生长表现出更好的益生元活性。超声可以有效地应用于 FVP 的降解，以改善其理化性质和生物活性。
