Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran and Faculty of Nutrition and Food Science, Tabriz University of Medical Science, Tabriz, Iran.
Trita Nanomedicine Research Center, Trita Pharmaceuticals, Zanjan, Iran.
Carbohydr Polym. 2022 Feb 15;278:118919. doi: 10.1016/j.carbpol.2021.118919. Epub 2021 Nov 22.
α-Amylase encapsulated in water in oil (W/O) emulsion was prepared using poly ethylene glycol (PEG 10000) (2%w/v) as water phase and ethylcellulose (EC) in ethyl acetate as oil phase at the ratio of 10:90 v/v. Next, the electrospun fibers were prepared by mixing enzyme loaded emulsion with EC solution (20%w/v) in acetic acid/ethyl acetate (20:80 v/v) at the 2:1 ratio. The emulsion showed good physical stability. The immobilized enzyme showed high activity across a board range of pHs and temperatures. The storage stability of the immobilized enzyme was 2 fold of free enzyme activity after 45 days. The residual activity of immobilized α-amylase onto of fibers after 10 and 15 repeated cycles, was approximately 100% and 50%, respectively. The results of this study indicated that the α-amylase loaded EC fibers have acceptable activity against harsh conditions and excellent reusability.
将聚乙二醇（PEG10000）（2%w/v）作为水相，乙基纤维素（EC）在乙酸乙酯中作为油相，以 10:90v/v 的比例制备包埋在油包水（W/O）乳液中的α-淀粉酶。然后，将负载酶的乳液与在乙酸/乙酸乙酯（20:80v/v）中的 EC 溶液（20%w/v）以 2:1 的比例混合，制备出电纺纤维。乳液表现出良好的物理稳定性。固定化酶在广泛的 pH 和温度范围内表现出高活性。固定化酶的储存稳定性在 45 天后是游离酶活性的 2 倍。在 10 次和 15 次重复循环后，纤维上固定化α-淀粉酶的剩余活性分别约为 100%和 50%。这项研究的结果表明，负载α-淀粉酶的 EC 纤维具有良好的耐苛刻条件的活性和优异的可重复使用性。
