Zhu Jing, Sun Xiang, Tang Qi-Yong, Zhang Zhi-Dong
Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Xinjiang Laboratory of Special Environmental Microbiology, Urumqi, China.
School of Life Sciences, Hebei University, Baoding, China.
Front Microbiol. 2021 Dec 16;12:778327. doi: 10.3389/fmicb.2021.778327. eCollection 2021.
Endophytes are essential components of plant microbiota. Studies have shown that environmental factors and seasonal alternation can change the microbial community composition of plants. However, most studies have mainly emphasized the transitive endophyte communities and seasonal alternation but paid less attention to their persistence through multiple seasons. is a perennial halophyte growing in an arid habitat with radiation stress (Cs) in northwest China. In this study, growing under different environmental stresses were selected to investigate the dynamics and persistency of endophytic microbial communities amid seasons in a year. The results showed that Gammaproteobacteria and unassigned Actinobacteria were the most dominant bacterial communities, while the most dominant fungal communities were Dothideomycetes, unassigned Fungi, and Sodariomycetes. The bacterial community diversity in roots was higher than that in aerial tissues, and root communities had higher diversity in summer and autumn. In contrast, the fungal community diversity was higher in aerial tissues comparing to roots, and the highest diversity was in spring. Season was a determinant factor in the microbial community composition in the roots but not in the aerial tissues. RaupCrick index suggested that the bacterial communities were mainly shaped by stochastic processes. Our research investigated the community traits and members with temporal persistency. For example, bacterial taxa , , , Xanthomonadaceae_B_OTU_211, and fungal taxa F_OTU_388, F_OTU_404, F_OTU_445, and unassigned Fungi F_OTU_704, F_OTU_767 showed higher frequencies than predicted in all the four seasons tested with neutral community model. The networks of co-occurrence associations presented in two or more seasons were visualized which suggested potential time-continuous core modules in most communities. In addition, the community dynamics and persistency also showed different patterns by radiation levels. Our findings would enhance our understanding of the microbial community assembly under environmental stress, and be promising to improve the development of integrated concept of core microbiome in future.
内生菌是植物微生物群的重要组成部分。研究表明，环境因素和季节交替会改变植物的微生物群落组成。然而，大多数研究主要强调了过渡性内生菌群落和季节交替，而对它们在多个季节中的持续性关注较少。[植物名称]是一种生长在中国西北部干旱生境且遭受辐射胁迫（Cs）的多年生盐生植物。在本研究中，选取了在不同环境胁迫下生长的[植物名称]来调查内生微生物群落在一年不同季节中的动态变化和持续性。结果表明，γ-变形菌纲和未分类的放线菌是最主要的细菌群落，而最主要的真菌群落是座囊菌纲、未分类的真菌和粪壳菌纲。根部的细菌群落多样性高于地上组织，且根部群落在夏季和秋季具有更高的多样性。相比之下，地上组织中的真菌群落多样性高于根部，且春季的多样性最高。季节是根部微生物群落组成的决定性因素，但对地上组织而言并非如此。劳普-克里克指数表明细菌群落主要受随机过程影响。我们的研究调查了具有时间持续性的群落特征和成员。例如，细菌分类单元[具体分类单元1]、[具体分类单元2]、[具体分类单元3]、黄单胞菌科_B_OTU_211，以及真菌分类单元F_OTU_388、F_OTU_404、F_OTU_445和未分类的真菌F_OTU_704、F_OTU_767在所有四个测试季节中的出现频率均高于中性群落模型预测值。展示了在两个或更多季节中出现的共现关联网络，这表明大多数群落中存在潜在的时间连续核心模块。此外，群落动态和持续性也因辐射水平呈现出不同模式。我们的研究结果将增进我们对环境胁迫下微生物群落组装的理解，并有望在未来推动核心微生物组综合概念的发展。
