The Department of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin, Heilongjiang, China.
Oxid Med Cell Longev. 2021 Dec 24;2021:3673313. doi: 10.1155/2021/3673313. eCollection 2021.
This study investigated whether the erythrocyte fraction in thrombi would be increased with serum iron via oxidative stress.
This study retrospectively enrolled patients with acute ischemic stroke treated using endovascular treatment in a single stroke center from October to December 2019. We examined the relationship between serum iron and erythrocyte-rich thrombi and the correlation of serum iron and the erythrocyte fraction in thrombi using clinical samples. Experiments and were performed to investigate the influence of oxidative stress on the correlation between serum iron concentration and erythrocyte fraction in thrombi.
We found from the clinical samples that serum iron concentration was related to erythrocyte-rich thrombi and positively associated with the erythrocyte fraction in thrombi . Further, the tightness of the fibrin networks regulating the erythrocyte fraction in thrombi was increased with serum iron concentration . Additionally, the oxidative stress level was increased with serum iron concentration . Moreover, we found that the tightness of the fibrin networks increased with higher oxidative stress levels . Lastly, experiments with inhibiting oxidative stress showed that the erythrocyte fraction in thrombi and the tightness of fibrin networks significantly increased in the iron group than those in the iron with oxidative stress inhibitor group and control group.
Oxidative stress played a role in the process that the erythrocyte fraction in thrombi was increased with serum iron by influencing fibrin networks.
本研究旨在探讨氧化应激是否会通过血清铁增加血栓中的红细胞部分。
本研究回顾性纳入了 2019 年 10 月至 12 月在单中心卒中中心接受血管内治疗的急性缺血性卒中患者。我们使用临床样本研究了血清铁与富含红细胞的血栓之间的关系，以及血清铁与血栓中红细胞部分的相关性。实验 1 和实验 2 研究了氧化应激对血清铁浓度与血栓中红细胞部分相关性的影响。
我们从临床样本中发现，血清铁浓度与富含红细胞的血栓有关，并且与血栓中的红细胞部分呈正相关。此外，调节血栓中红细胞部分的纤维蛋白网络的紧密性随着血清铁浓度的增加而增加。此外，氧化应激水平随着血清铁浓度的增加而升高。此外，我们发现纤维蛋白网络的紧密性随着更高的氧化应激水平而增加。最后，实验 1 抑制氧化应激表明，与铁加氧化应激抑制剂组和对照组相比，铁组的血栓中红细胞部分和纤维蛋白网络的紧密性显著增加。
氧化应激通过影响纤维蛋白网络，在血清铁增加血栓中红细胞部分的过程中发挥作用。
