Darijani Atefeh, Arefkhah Nasir, Shahriarirad Sepehr, Zoghi Sina, Namavari Mehdi, Moshfe Abdolali, Zaraei Marzieh, Sarkari Bahador
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
J Parasitol Res. 2021 Dec 24;2021:4258513. doi: 10.1155/2021/4258513. eCollection 2021.
Among the protozoa of veterinary importance, is responsible for large economic and productive losses in cattle herds. Dogs are being considered the definitive hosts of the parasite. has gained considerable attention through its role in the etiology of bovine abortion. The current study aimed at assessing the status of infection in cattle in Boyer-Ahmad County in Kohgiluyeh and Boyer-Ahmad province, southwest of Iran.
In this cross-sectional study, 150 cattle blood samples were collected and samples were screened for antibodies using a modified direct agglutination test (MAT). For the same samples, 130 buffy coats were collected and tested, by PCR, for the presence of DNA, targeting the Nc-5 gene.
Anti- antibodies were detected in the sera of 49 out of 150 cattle which is corresponding to a seroprevalence rate of 32.7%. DNA was detected in buffy coats of 26 out of 127 (20.47%) cattle. Even though infection was more common in females and in five-year-old cattle by serology and molecular methods, yet there was no statistically significant difference between age, sex, and infection in both molecular and serological methods ( > 0.05).
Findings of the current study indicate a high rate of infection in cattle of Boyer-Ahmad region in the southwest of Iran. This issue should be further investigated and the prevention and control of this parasite in livestock, due to the high financial burden of this parasite in the livestock industry, should be considered.
在具有兽医重要性的原生动物中，[寄生虫名称]对牛群造成了巨大的经济和生产损失。狗被认为是这种寄生虫的终末宿主。[寄生虫名称]因其在牛流产病因学中的作用而受到了相当大的关注。本研究旨在评估伊朗西南部科吉卢耶-博耶艾哈迈德省博耶艾哈迈德县牛群中[寄生虫名称]感染的状况。
在这项横断面研究中，采集了150份牛血样本，并使用改良直接凝集试验（MAT）对样本进行[寄生虫名称]抗体筛查。对于相同的样本，采集了130份血沉棕黄层，并通过聚合酶链反应（PCR）检测针对Nc - 5基因的[寄生虫名称]DNA的存在情况。
在150头牛中的49头牛血清中检测到抗[寄生虫名称]抗体，血清阳性率为32.7%。在127头牛中的26头牛（20.47%）的血沉棕黄层中检测到[寄生虫名称]DNA。尽管通过血清学和分子方法检测到[寄生虫名称]感染在雌性和五岁牛中更为常见，但在分子和血清学方法中，年龄、性别与[寄生虫名称]感染之间均无统计学显著差异（P > 0.05）。
本研究结果表明伊朗西南部博耶艾哈迈德地区的牛群中[寄生虫名称]感染率很高。由于这种寄生虫给畜牧业带来高昂的经济负担，这个问题应进一步调查，并应考虑对家畜中这种寄生虫的预防和控制。
