Department of Comparative Immunobiology, Zoological Institute, Christian-Albrechts University, Kiel, Germany.
Front Cell Infect Microbiol. 2021 Dec 17;11:775634. doi: 10.3389/fcimb.2021.775634. eCollection 2021.
In comparison with the standard monoxenic maintenance in the laboratory, rearing the nematode on its natural microbiota improves its fitness and immunity against pathogens. Although is known to exhibit choice behavior and pathogen avoidance behavior, little is known about whether actively chooses its (beneficial) microbiota and whether the microbiota influences worm behavior. We examined eleven natural isolates in a multiple-choice experiment for their choice behavior toward four natural microbiota bacteria and found that microbiota choice varied among isolates. The natural isolate MY2079 changed its choice behavior toward microbiota isolate MYb71 in both multiple-choice and binary-choice experiments, in particular on proliferating bacteria: MYb71 was chosen less than other microbiota bacteria or OP50, but only after preconditioning with MYb71. Examining escape behavior and worm fitness on MYb71, we ruled out pathogenicity of MYb71 and consequently learned pathogen avoidance behavior as the main driver of the behavioral change toward MYb71. The change in behavior of MY2079 toward microbiota bacterium MYb71 demonstrates how the microbiota influences the worm's choice. These results might give a baseline for future research on host-microbiota interaction in the model.
与实验室标准的单养维持相比，在自然微生物群上饲养线虫可以提高其对病原体的适应性和免疫力。虽然已知线虫表现出选择行为和病原体回避行为，但对于线虫是否主动选择其（有益的）微生物群，以及微生物群是否影响线虫行为，人们知之甚少。我们在多项选择实验中检查了 11 种天然分离物对四种天然微生物群细菌的选择行为，发现微生物群的选择在分离物之间存在差异。天然分离物 MY2079 在多项选择和双项选择实验中改变了对微生物群分离物 MYb71 的选择行为，特别是在增殖细菌上：与其他微生物群细菌或 OP50 相比，选择 MYb71 的比例较低，但仅在经过 MYb71 预处理后才会这样。在研究 MYb71 上的逃避行为和线虫适应性后，我们排除了 MYb71 的致病性，因此将回避病原体行为作为线虫对 MYb71 行为变化的主要驱动因素。MY2079 对线虫微生物群细菌 MYb71 行为的变化表明了微生物群如何影响线虫的选择。这些结果可能为未来在秀丽隐杆线虫模型中研究宿主-微生物群相互作用提供一个基线。
