用于 中跨代习得病原体回避行为分析的方案。
Protocol for transgenerational learned pathogen avoidance behavior assays in .
机构信息
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
LSI Genomics, Princeton University, Princeton, NJ 08544, USA.
出版信息
STAR Protoc. 2021 Mar 10;2(1):100384. doi: 10.1016/j.xpro.2021.100384. eCollection 2021 Mar 19.
Animal experiences, including learned behaviors, can be passed down to several generations of progeny in a phenomenon known as transgenerational epigenetic inheritance. Yet, little is known regarding the molecular mechanisms regulating physiologically relevant transgenerational memories. Here, we present a method for in which worms learn to avoid the pathogen (PA14). Unlike previous protocols, this training paradigm, either using PA14 lawns or through exposure to a PA14 small RNA (P11), induces memory in four generations of progeny. For complete details on the use and execution of this protocol, please refer to Moore et al. (2019) and Kaletsky et al. (2020).
动物的经验，包括习得行为，可通过一种被称为跨代表观遗传遗传的现象传递给几代后代。然而，关于调节生理相关跨代记忆的分子机制知之甚少。在这里，我们提出了一种方法，通过该方法，线虫学会了避免病原体 （PA14）。与以前的方案不同，这种训练模式，无论是使用 PA14 草坪还是通过暴露于 PA14 小 RNA（P11），都会在四代后代中诱导记忆。有关该方案的使用和执行的完整详细信息，请参阅 Moore 等人（2019 年）和 Kaletsky 等人（2020 年）。
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