Anticolonization of Carbapenem-Resistant by LP1812 Through Accumulated Acetic Acid in Mice Intestinal.

Carbapenem-resistant (CRKP) is highly prevalent and poses a significant threat to public health. In critically ill patients, gut colonization is considered to be the reservoir of recurrent CRKP infection. Therefore, eliminating CRKP carriage in the intestine is critical for preventing subsequent CRKP infection. In the present study, LP1812, a probiotic that can inhibit CRKP , was used as a candidate probiotic to investigate its efficacy for CRKP anticolonization. Compared with the control, mice fed with 1×10 CFU LP1812 exhibited significant CRKP clearance from 1×10 CFU/mg to less than 10 CFU/mg in mice feces. Furthermore, 16S RNA gene sequencing revealed that LP1812 modulated mice microbiota by increasing the relative abundance of the genus , , and . Further KEGG pathway enrichment analysis revealed that fatty acid-utilizing bacteria, such as acetate-producing and flourished in mice fed with LP1812. Moreover, we found that the concentration of acetic acid was higher in LP1812, which inhibited the growth of strains . Meanwhile, mice intragastrically administered with acetic acid exhibited significantly increased CRKP elimination . In conclusion, LP1812 is a potential candidate for intestinal CRKP anticolonization by regulating the intestinal microbiota and inhibiting CRKP increased acetic acid in the intestinal lumen.