Qiao Huanhuan, Wu Jieting, Zhang Xiaodong, Luo Jian, Wang Hao, Ming Dong
Functional Materials Laboratory, Institute of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China.
Palo Alto Veterans Institute for Research, VA Palo Alto Health Care System, Palo Alto, CA, United States.
Front Chem. 2021 Dec 16;9:786354. doi: 10.3389/fchem.2021.786354. eCollection 2021.
The study of different genes, chromosomes and the spatiotemporal relationship between them is of great significance in the field of biomedicine. CRISPR-Cas9 has become the most widely used gene editing tool due to its excellent targeting ability. In recent years, a series of advanced imaging technologies based on Cas9 have been reported, providing fast and convenient tools for studying the sites location of genome, RNA, and chromatin. At the same time, a variety of CRISPR-Cas9-based imaging systems have been developed, which are widely used in real-time multi-site imaging . In this review, we summarized the component and mechanism of CRISPR-Cas9 system, overviewed the NIR imaging and the application of NIR fluorophores in the delivery of CRISPR-Cas9, and highlighted advances of the CRISPR-Cas9-based imaging system. In addition, we also discussed the challenges and potential solutions of CRISPR-Cas9-based imaging methods, and looked forward to the development trend of the field.
研究不同的基因、染色体以及它们之间的时空关系在生物医学领域具有重要意义。CRISPR-Cas9因其出色的靶向能力已成为应用最为广泛的基因编辑工具。近年来，一系列基于Cas9的先进成像技术被报道，为研究基因组、RNA和染色质的位点定位提供了快速便捷的工具。同时，多种基于CRISPR-Cas9的成像系统也已被开发出来，并广泛应用于实时多位点成像。在本综述中，我们总结了CRISPR-Cas9系统的组成和机制，概述了近红外成像以及近红外荧光团在CRISPR-Cas9递送中的应用，并重点介绍了基于CRISPR-Cas9的成像系统的进展。此外，我们还讨论了基于CRISPR-Cas9的成像方法所面临的挑战和潜在解决方案，并展望了该领域的发展趋势。
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