用于研究颅内压升高时血液动力学反应的闭合颅窗啮齿动物模型。
Closed cranial window rodent model for investigating hemodynamic response to elevated intracranial pressure.
机构信息
Department of Biomedical Engineering Carnegie Mellon University Pittsburgh Pennsylvania USA.
Department of Anesthesiology Allegheny-Singer Research Institute Pittsburgh Pennsylvania USA.
出版信息
Animal Model Exp Med. 2021 Nov 19;4(4):391-397. doi: 10.1002/ame2.12187. eCollection 2021 Dec.
BACKGROUND
Elevated intracranial pressure (ICP) occurs in several physiological and pathological conditions, yet long-term sequellae are not common, which implies that blood flow is preserved above ischemic thresholds.
METHODS
This pilot study sought to confirm this hypothesis using a closed cranial window model in a rat in which ICP was elevated to 120 mmHg for 12 min, and superficial cortical perfusion was measured by laser Doppler flowmetry and laser speckle flowmetry.
RESULTS
Following a transient increase, cortical blood flow decreased to between 25% and 75% of baseline. These levels correspond to disrupted metabolism and decreased protein synthesis but did not exceed thresholds for electrical signaling or membrane integrity. This may partially explain how some episodes of elevated ICP remain benign.
CONCLUSION
The closed cranial window model provides a platform for prospective study of physiologic responses to artificially elevated ICP during neurosurgery to promote hemostasis.
背景
颅内压升高（ICP）发生在几种生理和病理情况下，但长期后遗症并不常见，这意味着血流在缺血阈值以上得以保持。
方法
本研究使用大鼠闭合颅窗模型来验证这一假说，在该模型中，ICP 升高至 120mmHg 持续 12 分钟，并通过激光多普勒血流仪和激光散斑血流仪测量浅表皮质灌注。
结果
短暂升高后，皮质血流降至基线的 25%至 75%之间。这些水平对应于代谢紊乱和蛋白质合成减少，但未超过电信号或膜完整性的阈值。这可能部分解释了为什么一些 ICP 升高的发作仍然是良性的。
结论
闭合颅窗模型为前瞻性研究神经外科中人为升高 ICP 时的生理反应提供了一个平台，以促进止血。
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