Francino-Urdaniz Irene M, Whitehead Timothy A
Department of Chemical and Biological Engineering, University of Colorado JSC Biotechnology Building, 3415 Colorado Avenue Boulder CO 80305 USA
RSC Chem Biol. 2021 Sep 29;2(6):1580-1589. doi: 10.1039/d1cb00169h. eCollection 2021 Dec 2.
This mini-review presents a critical survey of techniques used for epitope mapping on the SARS-CoV-2 Spike protein. The sequence and structures for common neutralizing and non-neutralizing epitopes on the Spike protein are described as determined by X-ray crystallography, electron microscopy and linear peptide epitope mapping, among other methods. An additional focus of this mini-review is an analytical appraisal of different deep mutational scanning workflows for conformational epitope mapping and identification of mutants on the Spike protein which escape antibody neutralization. Such a focus is necessary as a critical review of deep mutational scanning for conformational epitope mapping has not been published. A perspective is presented on the use of different epitope determination methods for development of broadly potent antibody therapies and vaccines against SARS-CoV-2.
本综述对用于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突蛋白表位作图的技术进行了批判性审视。除其他方法外，还描述了通过X射线晶体学、电子显微镜和线性肽表位作图确定的刺突蛋白上常见中和及非中和表位的序列和结构。本综述的另一个重点是对不同深度突变扫描工作流程进行分析评估，以用于构象表位作图以及鉴定刺突蛋白上逃避抗体中和的突变体。由于尚未发表对用于构象表位作图的深度突变扫描的批判性综述，因此有必要进行这样的重点研究。本文还就使用不同表位测定方法开发针对SARS-CoV-2的广泛有效的抗体疗法和疫苗提出了观点。
