Otmani Ines, Abdennour Cherif, Dridi Amina, Kahalerras Labiba, Halima-Salem Abdelaziz
Department of Biology, Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Badji Mokhtar-Annaba, Annaba, Algeria.
Department of Biology, Laboratory of Plant Biology and Environment, Faculty of Sciences, University of Badji Mokhtar-Annaba, Annaba, Algeria.
Vet World. 2019;12(4):551-557. doi: 10.14202/vetworld.2019.551-557. Epub 2019 Apr 17.
This study aimed to compare the physicochemical, the biochemical, and the antioxidant characteristics of unifloral bitter honey and polyfloral sweet honey.
Unifloral bitter and polyfloral sweet honey samples were, respectively, harvested in January and July, and then, they were extracted by the traditional method. The markers of refractive index, pH, free acidity, Brix index, density, reducing sugars, total polyphenols, flavonoids, tannins, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing/antioxidant power (FRAP) were evaluated.
The obtained results showed that the physicochemical parameters are within the normal ranges, in which they meet the international standards (Codex Alimentarius). For biochemical constituents, matching concentrations of reducing sugars (glucose+fructose) were observed in both samples, while that of sucrose were very low in unifloral than polyfloral honey. The levels of the active ingredients showed a difference in total polyphenols and tannins of the two types of honey studied, whereas that of flavonoids were almost similar. The antioxidant activity of various samples evaluated by DPPH and FRAP indicated that unifloral has a superior activity.
Compared to polyfloral honey, unifloral bitter honey has lower sucrose, high total polyphenols, and tannins levels, in addition to higher antioxidant potential.
本研究旨在比较单花种苦蜂蜜和多花种甜蜂蜜的物理化学、生化及抗氧化特性。
单花种苦蜂蜜和多花种甜蜂蜜样本分别于1月和7月采集,然后采用传统方法进行提取。对折射率、pH值、游离酸度、白利糖度指数、密度、还原糖、总多酚、黄酮类化合物、单宁、2,2-二苯基-1-苦基肼(DPPH)和铁还原/抗氧化能力(FRAP)等指标进行了评估。
所得结果表明,物理化学参数在正常范围内,符合国际标准(食品法典)。对于生化成分,两个样本中还原糖(葡萄糖+果糖)的浓度相当,而单花种蜂蜜中的蔗糖浓度比多花种蜂蜜低得多。所研究的两种蜂蜜中,活性成分的含量在总多酚和单宁方面存在差异,而黄酮类化合物的含量几乎相似。通过DPPH和FRAP评估的各种样本的抗氧化活性表明,单花种蜂蜜具有更高的活性。
与多花种蜂蜜相比,单花种苦蜂蜜的蔗糖含量较低,总多酚和单宁含量较高,且具有更高的抗氧化潜力。