College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, P. R. China.
Department of Allergy, The Affiliated Hospital of Qingdao University, No. 16, Jiangsu Road, Qingdao, Shandong Province 266003, P. R. China.
J Agric Food Chem. 2022 Jan 12;70(1):360-372. doi: 10.1021/acs.jafc.1c05421. Epub 2022 Jan 3.
Marine molluscs are seafood consumed worldwide and could cause food allergies, while investigation on their sensitizing components and cross-reactivity seems to be rare. Furthermore, allergy to mites may result in anaphylaxis in mollusc-allergic individuals owing to their cross-reactivity. The aim of the study was to identify cross-reactive allergens and investigate the cross-reactivity between different mollusc groups and mite-mollusc. The extracted mollusc and dust mite proteins were separated by SDS-PAGE, and IgE-binding components were recognized by immunoblotting with sera from patients sensitized to mollusc and mite. Cross-reactivity of different mollusc groups and mite-mollusc was assessed using ELISA and inhibition ELISA. The results of the immune detection, ELISA, and inhibition ELISA indicated that different mollusc groups and mite-mollusc showed varying degrees of cross-reactivity. The most frequently recognized cross-reactive protein was paramyosin from different mollusc groups and dust mite, while cross-reactive allergen paramyosin in the mite extract was identified and evaluated by MS and Allermatch, respectively. Inhibition ELISA studies also revealed that paramyosin played an important role in molluscan and mite-molluscan cross-reactivity. These findings contribute to a better understanding of the cross-reactivity involving mollusc species and mite-mollusc, which can be used to assist in the diagnosis and treatment of mite- and mollusc-allergic disorders.
海洋贝类是全球范围内食用的海鲜，可能引起食物过敏，而对其致敏成分和交叉反应的研究似乎很少。此外，由于螨类和贝类之间的交叉反应，螨类过敏可能导致贝类过敏个体发生过敏反应。本研究旨在鉴定交叉反应性过敏原，并研究不同贝类组与螨-贝类之间的交叉反应性。通过 SDS-PAGE 分离贝类和尘螨蛋白，并用贝类和尘螨过敏患者的血清通过免疫印迹识别 IgE 结合成分。使用 ELISA 和抑制 ELISA 评估不同贝类组和螨-贝类之间的交叉反应性。免疫检测、ELISA 和抑制 ELISA 的结果表明，不同贝类组和螨-贝类之间表现出不同程度的交叉反应性。不同贝类组和尘螨的原肌球蛋白是最常被识别的交叉反应蛋白，而从螨提取物中鉴定并通过 MS 和 Allermatch 评估了交叉反应性过敏原原肌球蛋白。抑制 ELISA 研究还表明，原肌球蛋白在贝类和螨-贝类的交叉反应中起着重要作用。这些发现有助于更好地了解涉及贝类物种和螨-贝类的交叉反应性，这可用于辅助螨类和贝类过敏疾病的诊断和治疗。
