Johansson E, Aponno M, Lundberg M, van Hage-Hamsten M
Department of Medicine, Unit of Clinical Immunology and Allergy, Karolinska Institutet and Hospital, Stockholm, Sweden.
Allergy. 2001 Jul;56(7):660-6. doi: 10.1034/j.1398-9995.2001.00798.x.
The nematode Anisakis simplex is a common parasite on fish and other seafood. It is considered to be a food allergen and to induce IgE-mediated reactions. Allergenic cross-reactivity between A. simplex and other nematodes has been reported, as has cross-reactivity with arthropods: red mosquito larvae and German cockroach. We have here studied the allergenic relationship between A. simplex and four different dust-mite species.
Serum samples collected from 69 farmers allergic to dust mites were analyzed for IgE to A. simplex by CAP FEIA. Allergenic cross-reactivity between A. simplex and dust mites was studied in two of the sera by CAP FEIA and immunoblotting inhibition.
We found that 14/69 farmers had detectable levels of IgE antibodies to A. simplex. The IgE response in CAP FEIA to A. simplex was inhibited to various degrees in the two studied sera by extracts of the dust mites Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Dermatophagoides pteronyssinus. In the reverse inhibition experiment, extract of A. simplex inhibited the response in both sera to A. siro and T. putrescentiae, but not to L. destructor. The IgE binding to D. pteronyssinus was inhibited in one of the two sera. In blotting inhibition experiments, the IgE binding to several allergens in A. simplex was inhibited by each of the four mite extracts, especially by A. siro and T. putrescentiae, which completely inhibited the IgE binding to several allergens.
The results show allergenic cross-reactivity between several allergens in A. simplex and four dust-mite species. The clinical significance of this cross-reactivity remains to be evaluated.
简单异尖线虫是鱼类和其他海产品上常见的寄生虫。它被认为是一种食物过敏原，可引发IgE介导的反应。已报道简单异尖线虫与其他线虫之间存在变应原交叉反应，与节肢动物（红蚊虫幼虫和德国小蠊）也存在交叉反应。我们在此研究了简单异尖线虫与四种不同尘螨种类之间的变应原关系。
收集69名对尘螨过敏的农民的血清样本，通过CAP FEIA分析其中针对简单异尖线虫的IgE。通过CAP FEIA和免疫印迹抑制法，在两份血清中研究简单异尖线虫与尘螨之间的变应原交叉反应。
我们发现，69名农民中有14名的血清中可检测到针对简单异尖线虫的IgE抗体。在两份研究血清中，粉尘螨、腐食酪螨、粗脚粉螨和屋尘螨的提取物对CAP FEIA中针对简单异尖线虫的IgE反应有不同程度的抑制。在反向抑制实验中，简单异尖线虫提取物抑制了两份血清中对粉尘螨和腐食酪螨的反应，但未抑制对粗脚粉螨的反应。两份血清中的一份中，屋尘螨的IgE结合受到抑制。在印迹抑制实验中，四种螨提取物中的每一种都抑制了简单异尖线虫中几种变应原的IgE结合，尤其是粉尘螨和腐食酪螨，它们完全抑制了对几种变应原的IgE结合。
结果显示简单异尖线虫中的几种变应原与四种尘螨种类之间存在变应原交叉反应。这种交叉反应的临床意义仍有待评估。
