State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
Gene. 2022 Mar 20;815:146138. doi: 10.1016/j.gene.2021.146138. Epub 2021 Dec 31.
Cotton is an important textile industry raw material crops, which plays a critical role in the development of society. MADS transcription factors (TFs) play a key role about the flowering time, flower development, and abiotic stress responses in plants, but little is known about their functions on abiotic stress in cotton. In this study, a MIKC subfamily gene from cotton, GhFYF (FOREVER YOUNG FLOWER), was isolated and characterized. Our data showed that GhFYF localized to the nucleus. A β-glucuronidase (GUS) activity assay revealed that the promoter of GhFYF was mainly expressed in the flower and seed of ProGhFYF::GUS transgenic A. thaliana plants. The GUS staining of flowers and seeds was deepened after drought, salt treatment, and the expression level of the GUS gene and corresponding stress genes AtERD10, AtAnnexin1 are up-regulated in the inflorescence. Overexpression GhFYF in A. thaliana could promote the seed germination and growth under different salt concentrations, and determin the proline content. Yeast two-hybrid (Y2H) assays showed that GhFYF interacted with the HAD-like protein GhGPP2, which has responds to abiotic stress. Our findings indicate that GhFYF is involved in abiotic stress responses, especially for salt stress. This work establishes a solid foundation for further functional analysis of the GhFYF gene in cotton.
棉花是一种重要的纺织工业原料作物，在社会发展中起着关键作用。MADS 转录因子（TFs）在植物的开花时间、花发育和非生物胁迫响应中起着关键作用，但关于它们在棉花中的非生物胁迫功能知之甚少。本研究从棉花中分离和鉴定了一个 MIKC 亚家族基因 GhFYF（永远年轻的花）。我们的数据表明，GhFYF 定位于细胞核。β-葡萄糖醛酸酶（GUS）活性测定表明，GhFYF 启动子主要在 ProGhFYF::GUS 转基因拟南芥植物的花和种子中表达。干旱、盐处理后，花和种子的 GUS 染色加深，花序中 GUS 基因和相应胁迫基因 AtERD10、AtAnnexin1 的表达水平上调。在拟南芥中过表达 GhFYF 可以促进不同盐浓度下种子的萌发和生长，并决定脯氨酸的含量。酵母双杂交（Y2H）试验表明 GhFYF 与 HAD 样蛋白 GhGPP2 相互作用，GhGPP2 对非生物胁迫有响应。我们的研究结果表明，GhFYF 参与非生物胁迫响应，特别是盐胁迫。这项工作为进一步分析棉花中 GhFYF 基因的功能奠定了基础。
