Snow E C
J Immunol. 1987 Sep 15;139(6):1758-62.
Hapten-specific, trinitrophenyl antigen-binding B cells (TNP-ABC) were purified from inbred strains of mice representative of short-, intermediate-, and long-lived animals. Such populations of B cells were stimulated by either thymus-independent or thymus-dependent antigens in vitro and evaluated for both proliferation and differentiation into antibody-secreting cells. In the thymus-dependent system, the three strains of mice were selected on the basis of all being able to interact appropriately with the same T helper cell line. The results indicate that TNP-ABC purified from aged animals of all three strains responded to both forms of antigenic stimulation similar to TNP-ABC selected from young, littermate control animals. These results are discussed in terms of concepts of intrinsic B cell defects during the aging process.
从代表短寿命、中等寿命和长寿命动物的近交系小鼠中纯化出对特定半抗原三硝基苯基抗原具有结合能力的B细胞（TNP-ABC）。将此类B细胞群体在体外用非胸腺依赖性或胸腺依赖性抗原刺激，并评估其增殖情况以及分化为抗体分泌细胞的能力。在胸腺依赖性系统中，选择这三种品系的小鼠是基于它们都能够与同一T辅助细胞系进行适当相互作用。结果表明，从所有这三个品系的老年动物中纯化出的TNP-ABC，对两种形式的抗原刺激的反应与从年轻的同窝对照动物中选出的TNP-ABC相似。根据衰老过程中内在B细胞缺陷的概念对这些结果进行了讨论。
