Kirkland T N, Sieckmann D G, Longo D L, Mosier D E
J Immunol. 1980 Apr;124(4):1721-6.
The ability of various cell populations to bind and present the thymus-independent antigen TNP-Ficoll to a responding cell population was assessed. The in vitro antibody response to TNP-Ficoll depends upon the presence of B lymphocytes and plastic-adherent accessory cells, but does not require T lymphocytes. Purified B cells were the most effective population in binding and presenting TNP-Ficoll, and adherent cells did not perform this function. Antigen binding and presentation was antigen specific and could be blocked with anti-mu antibody, but not by antibodies directed against other immunoglobulin classes. Spleen cells from mice genetically unresponsive to TNP-Ficoll (CBA/N X BALB/c F1 males) were equally effective as normal spleen cells in antigen binding and presentation. We conclude that the initial events in the induction of the antibody response involves antigen binding by B cells, and that subsequent activation of the subset of B cells that can respond to TNP-Ficoll proceeds either via B cell-B cell interaction or B cell-dependent transfer of antigen to macrophage-like cells.
评估了各种细胞群体结合并将胸腺非依赖性抗原TNP-Ficoll呈递给反应性细胞群体的能力。对TNP-Ficoll的体外抗体反应依赖于B淋巴细胞和塑料黏附辅助细胞的存在，但不需要T淋巴细胞。纯化的B细胞是结合和呈递TNP-Ficoll最有效的细胞群体，而黏附细胞不具备此功能。抗原结合和呈递具有抗原特异性，可被抗μ抗体阻断，但不能被针对其他免疫球蛋白类别的抗体阻断。对TNP-Ficoll基因无反应的小鼠（CBA/N×BALB/c F1雄性）的脾细胞在抗原结合和呈递方面与正常脾细胞同样有效。我们得出结论，抗体反应诱导的初始事件涉及B细胞对抗原的结合，并且随后能够对TNP-Ficoll作出反应的B细胞亚群的激活要么通过B细胞与B细胞的相互作用，要么通过B细胞依赖性地将抗原转移至巨噬细胞样细胞来进行。
