Anderson P, Insel R A, Porcelli S, Ward J I
J Infect Dis. 1987 Oct;156(4):582-90. doi: 10.1093/infdis/156.4.583.
In radioantigen-binding assays for antibody to Haemophilus influenzae type b capsular polysaccharide, an apparent antibody concentration [( (["Ab"]) is assigned to test sera on the basis of binding equivalence to dilutions of standard adult antiserum. In childrens' sera, ["Ab"] can disagree between laboratories and between intralaboratory assays using different preparations of radioantigen. Immunochemical variables causing disagreement include the dilution of test serum at which ["Ab"] is assigned and the concentration and size distribution of radioantigen. Binding-dilution curves of many childrens' sera displayed shallower slopes than did the standard, thus ["Ab"] increased with dilution, particularly in low-binding sera. An increase in radioantigen concentration also raised ["Ab"]. Using larger-sized radioantigen raised the binding in standard and childrens' sera comparably and thus would not directly affect ["Ab"] but would raise ["Ab"] indirectly by increasing the serum dilution. These results are theoretically predicted if the affinity of childrens' antibodies are lower than that of the standard.
在针对b型流感嗜血杆菌荚膜多糖抗体的放射抗原结合试验中，根据与标准成人抗血清稀释液的结合等效性，为检测血清指定一个表观抗体浓度[（["Ab"]）]。在儿童血清中，不同实验室之间以及使用不同放射性抗原制剂的实验室内测定之间，["Ab"]可能会出现差异。导致差异的免疫化学变量包括指定["Ab"]时检测血清的稀释度以及放射性抗原的浓度和大小分布。许多儿童血清的结合稀释曲线斜率比标准曲线更平缓，因此["Ab"]随稀释度增加，尤其是在低结合血清中。放射性抗原浓度的增加也会提高["Ab"]。使用较大尺寸的放射性抗原会使标准血清和儿童血清中的结合量同等增加，因此不会直接影响["Ab"]，但会通过增加血清稀释度间接提高["Ab"]。如果儿童抗体的亲和力低于标准抗体，这些结果在理论上是可以预测的。
