Laboratory of Veterinary Internal Medicine II, Nippon Veterinary and Life Science University, 1-7-1 Kyonan, Musashino, Tokyo 180-0023, Japan.
J Vet Med Sci. 2022 Feb 23;84(2):257-260. doi: 10.1292/jvms.21-0566. Epub 2021 Dec 30.
This study compared canine and feline fibroblast growth factor (FGF)-23 concentration measurements between automated chemiluminescence assay (CLEIA) and enzyme-linked immunosorbent assay (ELISA). Seventy serum samples each from dogs and cats were evaluated. FGF-23 measurements by CLEIA significantly correlated with those of ELISA in both dogs and cats. The Bland-Altman test showed that FGF-23 between CLEIA and ELISA had fixed and proportional biases, respectively, in both dogs and cats. Measurements by CLEIA were lower than those of ELISA, especially in higher serum FGF-23 concentrations. This study showed that FGF-23 concentrations in dogs and cats can be evaluated by automated CLEIA. However, FGF-23 cannot be directly compared between CLEIA and ELISA.
本研究比较了犬和猫的成纤维细胞生长因子（FGF）-23 浓度在自动化化学发光测定法（CLEIA）和酶联免疫吸附测定法（ELISA）之间的测量结果。分别评估了 70 份来自犬和猫的血清样本。犬和猫的 CLEIA 检测的 FGF-23 浓度与 ELISA 检测的 FGF-23 浓度均具有显著相关性。Bland-Altman 检验显示，犬和猫的 CLEIA 和 ELISA 之间的 FGF-23 检测值分别存在固定和比例偏差。CLEIA 检测的测量值低于 ELISA 检测的测量值，尤其是在血清 FGF-23 浓度较高的情况下。本研究表明，犬和猫的 FGF-23 浓度可以通过自动化 CLEIA 进行评估。然而，CLEIA 和 ELISA 之间的 FGF-23 不能直接进行比较。
