Oishi H, Katsuno M, Umemura T, Nishimura J, Motomura S, Ibayashi H
Leuk Res. 1987;11(8):699-704. doi: 10.1016/0145-2126(87)90005-1.
We examined CFU-S proliferation stimulator, which recruits stem cells in DNA synthesis, in conditioned media prepared from bone marrow cells of patients with regeneration hemopoiesis after chemotherapy induced hypoplasia. This activity was estimated by hydroxyurea sensitivity of CFU-S in mice, under conditions of incubation with human bone marrow conditioned medium (BMCM). We found that CFU-S proliferation stimulator was present to a considerable extent in human regenerating BMCM, but less so in normal BMCM and that the production fluctuated with change of hemopoietic states, in the same patient. This stimulator was heat-labile, trypsin-sensitive and mainly produced by adherent cells. This factor may possibly be involved in regulation of proliferation of stem cells in regenerating bone marrow in humans.
我们检测了集落形成单位脾细胞（CFU-S）增殖刺激因子，该因子可使处于DNA合成期的干细胞募集，检测是在化疗诱导造血功能减退后再生造血的患者骨髓细胞制备的条件培养基中进行的。在与人类骨髓条件培养基（BMCM）共同孵育的条件下，通过小鼠CFU-S对羟基脲的敏感性来评估这种活性。我们发现，CFU-S增殖刺激因子在人类再生BMCM中大量存在，但在正常BMCM中含量较少，并且在同一患者中，其产生会随着造血状态的变化而波动。这种刺激因子对热不稳定，对胰蛋白酶敏感，主要由贴壁细胞产生。该因子可能参与了人类再生骨髓中干细胞增殖的调控。
