小檗碱对癫痫小鼠模型中某些表观遗传、转录调控及炎症生物标志物的影响
Impact of Berberine on Some Epigenetic, Transcription Regulation and Inflammatory Biomarkers in a Mice Model of Epilepsy.
作者信息
Ghanem Heba Bassiony, Emam Marwa Nagy, Dareen Abdelaziz Mohammed Ali, Rania Nagi Abd-Ellatif
机构信息
Clinical laboratory sciences Department, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia.
Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
出版信息
Rep Biochem Mol Biol. 2021 Oct;10(3):362-372. doi: 10.52547/rbmb.10.3.362.
BACKGROUND
Epilepsy is one of the most widespread neurological disease worldwide. Status epilepticus (SE) is a life-threatening neurologic disorder. Neuroprotective approaches are increasingly to discover a promising therapy to manage epileptic disorders. This study aimed to assess the impact of berberine on some epigenetic, transcription regulation & inflammatory biomarkers in a mice model of epilepsy.
METHODS
This work was performed on; Group I: (control), Group II: berberine-treated control,Group III: epilepsy group, Group IV: berberine-treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1β (TGF-1β), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression.
RESULTS
Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group.
CONCLUSION
Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.
背景
癫痫是全球最普遍的神经系统疾病之一。癫痫持续状态（SE）是一种危及生命的神经系统疾病。神经保护方法越来越多地用于寻找治疗癫痫疾病的有效疗法。本研究旨在评估黄连素对癫痫小鼠模型中一些表观遗传、转录调控和炎症生物标志物的影响。
方法
本研究分为四组进行：第一组（对照组）、第二组（黄连素处理的对照组）、第三组（癫痫组）、第四组（黄连素处理的癫痫组）。对各组进行肿瘤生长因子-1β（TGF-1β）、缺氧诱导因子-1α（HIF-1α）、脑源性神经营养因子（BDNF）水平、组蛋白脱乙酰酶（HDAC）活性及神经元限制性沉默因子（NRSF）基因表达的评估。
结果
研究表明，癫痫组中HIF-1α、TGF-1β、HDAC活性及NRSF基因表达水平显著升高，而黄连素处理的癫痫组这些水平降低。癫痫组中BDNF水平显著降低，而黄连素处理的癫痫组中BDNF水平升高。
结论
我们的研究表明，黄连素在癫痫小鼠模型中通过对一些表观遗传、转录因子和炎症生物标志物的调节作用具有抗癫痫作用。
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