鸢尾素和癌胚抗原（CEA）作为胃癌和结直肠癌潜在的诊断生物标志物
Irisin and Carcinoembryonic Antigen (CEA) as Potential Diagnostic Biomarkers in Gastric and Colorectal Cancers.
作者信息
Abd Temur Ahmed, Aqeel Rashid Farah
机构信息
Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq.
出版信息
Rep Biochem Mol Biol. 2021 Oct;10(3):488-494. doi: 10.52547/rbmb.10.3.488.
BACKGROUND
Carcinoembryonic antigen (CEA) is a common gastrointestinal tumor biomarker. Irisin is adipo-myokines that has been suggested to have a potential role in cancer development. However, limited studies test irisin as biomarker in gastric and colorectal cancers. Therefore, this study aims to investigate whether CEA and irisin could be a potential diagnostic biomarker in gastric and colorectal cancer.
METHODS
A case-control study consists of 90 subjects, 21 gastric cancer patients, 49 colorectal cancer patients and 20 control. Serum CEA was detected by fluorescence immunoassay (FIA) kit. Serum irisin was determined by enzyme-linked immunosorbent assay (ELISA) kit.
RESULTS
Serum CEA increases significantly and serum irisin decreases significantly in gastric and colorectal cancer patients. According to Receiver Operating Characteristic (ROC) curve analysis, in gastric cancer, the area under curve of CEA is 1.00 (95% CI, 1.000-1.000, p< 0.0001). The diagnostic cut-off of CEA is< 3.08 ng/ml with %100 sensitivity and 100% specificity. The area under curve of irisin is 0.94 (95% CI, 0.8177-1.000, p< 0.0001). The cut-off of irisin is> 30.2 ng/ml with %90 sensitivity and 100%, specificity. In colorectal cancer, the area under curve of CEA is 0.99 (95% CI, 0.9866-1.000, p< 0.0001) and the diagnostic value< 2.6 ng/ml with %98 sensitivity and %100 specificity. The area under curve of irisin is 0.96 (95% CI, 0.9155-1.000, p< 0.0001). The diagnostic cut-off of irisin is> 41.9 ng/ml with 88.1sensitivity and 90.5 specificity.
CONCLUSION
CEA and irisin could be powerful potential diagnostic biomarkers which would be use for early detection of gastric and colorectal cancers.
背景
癌胚抗原（CEA）是一种常见的胃肠道肿瘤生物标志物。鸢尾素是一种脂肪肌动蛋白，已被认为在癌症发展中具有潜在作用。然而，将鸢尾素作为胃癌和结直肠癌生物标志物的研究有限。因此，本研究旨在探讨CEA和鸢尾素是否可能成为胃癌和结直肠癌的潜在诊断生物标志物。
方法
一项病例对照研究包括90名受试者，21名胃癌患者、49名结直肠癌患者和20名对照。血清CEA通过荧光免疫分析（FIA）试剂盒检测。血清鸢尾素通过酶联免疫吸附测定（ELISA）试剂盒测定。
结果
胃癌和结直肠癌患者血清CEA显著升高，血清鸢尾素显著降低。根据受试者工作特征（ROC）曲线分析，在胃癌中，CEA的曲线下面积为1.00（95%CI，1.000 - 1.000，p < 0.0001）。CEA的诊断临界值< 3.08 ng/ml，灵敏度为100%，特异性为100%。鸢尾素的曲线下面积为0.94（95%CI，0.8177 - 1.000，p < 0.0001）。鸢尾素的临界值> 30.2 ng/ml，灵敏度为90%，特异性为100%。在结直肠癌中，CEA的曲线下面积为0.99（95%CI，0.9866 - 1.000，p < 0.0001），诊断值< 2.6 ng/ml，灵敏度为98%，特异性为100%。鸢尾素的曲线下面积为0.96（95%CI，0.9155 - 1.000，p < 0.0001）。鸢尾素的诊断临界值> 41.9 ng/ml，灵敏度为88.1%，特异性为90.5%。
结论
CEA和鸢尾素可能是强大的潜在诊断生物标志物，可用于胃癌和结直肠癌的早期检测。
Zotero 插件