Department of Urology, Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, Jiangsu, 212001, China.
Department of Orthopaedic Surgery, Orthopaedic Institute, The First Affiliated Hospital, Medical College, Soochow University, Suzhou, Jiangsu, 215006, China.
J Mater Chem B. 2022 Apr 6;10(14):2584-2596. doi: 10.1039/d1tb02318g.
Urinary tract infection (UTI) represents one of the most common nosocomial infections, which is mainly related to indwelling catheters or stents. In addition to the formation of biofilms to reduce antibiotic sensitivity, the urease-producing bacteria can also increase urine pH, causing Ca and Mg deposition and finally catheter obstruction. The prevention of UTIs and its complication (, encrustation) thus is a great challenge in design of catheters and ureteral stents. In this work, a metal-catechol-assisted mussel chemistry (, dopamine self-polymerization) was employed for surface functionalization of commercially available catheters with antimicrobial peptides (AMP), for the purpose of long-term anti-infection and encrustation prevention. To improve the stability of the polydopamine coating on polymeric stents, we used Cu-coordinated dopamine self-polymerization. Then, a cysteine-terminated AMP was introduced on the polydopamine coating through Michael addition. We found that the Cu-coordinated polydopamine coating showed improved stability and antibacterial effect. The cytotoxicity test confirmed that the bioinspired antibacterial coating showed good biocompatibility and no obvious toxicity. The results confirmed that the stents with AMP could inhibit bacterial growth and biofilm formation, and finally reduce the deposition of struvite and hydroxyapatite crystals both and . We anticipate that this bioinspired strategy would develop a safe, stable and effective antibacterial coating on urinary tract medical devices for long-term bacterial inhibition and encrustation prevention.
尿路感染（UTI）是最常见的医院获得性感染之一，主要与留置导管或支架有关。除了形成生物膜降低抗生素敏感性外，产脲酶细菌还可以增加尿液 pH 值，导致 Ca 和 Mg 沉积，最终导致导管阻塞。因此，预防尿路感染及其并发症（如结石形成）是导管和输尿管支架设计的一大挑战。在这项工作中，采用金属-儿茶酚辅助贻贝化学（多巴胺自聚合）对市售导管进行抗菌肽（AMP）的表面功能化，以实现长期抗感染和防结石形成。为了提高聚多巴胺涂层在聚合物支架上的稳定性，我们使用 Cu 配位的多巴胺自聚合。然后，通过迈克尔加成将半胱氨酸末端 AMP 引入到聚多巴胺涂层上。我们发现 Cu 配位的聚多巴胺涂层表现出更好的稳定性和抗菌效果。细胞毒性试验证实，仿生抗菌涂层具有良好的生物相容性，无明显毒性。结果证实，载 AMP 的支架可以抑制细菌生长和生物膜形成，最终减少鸟粪石和羟基磷灰石晶体的沉积。我们预计，这种仿生策略将为尿路医疗器械开发出安全、稳定、有效的抗菌涂层，以实现长期的细菌抑制和防结石形成。
