Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2022;2407:115-154. doi: 10.1007/978-1-0716-1871-4_11.
Cord blood is a readily available source of hematopoietic stem and progenitor cells (HSPCs) which can be infected with HIV-1 in vitro to produce inducible latently infected cells for reactivation studies. Infected HSPCs can also be found in the setting of clinically undetectable viremia in vivo. Here we describe an in vitro infection model utilizing cord blood derived HSPCs, as well as methods for isolating and characterizing provirus from bone marrow HSPCs from suppressed patients.
脐带血是造血干细胞和祖细胞（HSPCs）的易得来源，可在体外感染 HIV-1，以产生可诱导的潜伏感染细胞用于再激活研究。在体内临床无法检测到病毒血症的情况下，也可以在 HSPC 中发现感染的 HSPCs。在这里，我们描述了一种利用脐带血来源的 HSPCs 的体外感染模型，以及从抑制患者的骨髓 HSPCs 中分离和表征前病毒的方法。
