College of Environmental Science and Engineering, Dalian Maritime University, No.1 Linghai Road, Dalian, Liaoning, China, 116026.
Department of Chemistry, Lishui University, Lishui 32300, China.
J Chromatogr A. 2022 Jan 25;1663:462759. doi: 10.1016/j.chroma.2021.462759. Epub 2021 Dec 18.
Molecularly imprinted polymer was constructed for the first time through dummy imprinting strategy with homopiperonylamine as dummy template. The prepared dummy molecularly imprinted polymer (DMIP) showed high class selectivity towards the most popular amphetamine-type stimulants (ATSs) such as methamphetamine, amphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxy-amphetamine, and 3,4-methylenedioxy-N-ethylamphetamine with the imprinting factors of 2.280∼3.698 and selectivity factors of 1.654∼3.698. Moreover, ATSs could be rapidly adsorbed from water with the equilibrium time within 5 min. Hydrogen-bonding interaction between the amino groups of ATSs and carboxy on DMIP could be dominated adsorption mechanism. DMIP was employed as solid phase extraction (SPE) sorbents. Under the optimum extraction conditions, the method using DMIP-based SPE and high performance liquid chromatography-tandem mass spectrometry showed good linearity in the range of 0.025∼1.00 μmol L, good repeatability (RSD 4.8∼8.6%, n = 5) and low limits of quantification (0.007∼0.200 ng mL, S/N = 10). Satisfactory recoveries (72.5∼120%) with low RSD values (<10%) were obtained for all targets viz. spiked coke carbonated drinks, beer and cocktail. Compared with other commercial SPE sorbents, DMIP exhibited lower matrix effect (ME) for coke, beer and cocktail with ME values of 101∼124%, 75.8∼80.2% and 103∼128%, respectively. The obtained results suggested that the developed DMIP materials could be a potential candidate for pretreatment of ATSs in alcoholic and nonalcoholic beverages.
首次通过模板分子印迹策略，以胡椒基丙胺为模板分子，构建了分子印迹聚合物。所制备的 dummy 分子印迹聚合物（DMIP）对最常见的苯丙胺类兴奋剂（ATS）具有高的类选择性，如甲基苯丙胺、苯丙胺、3,4-亚甲二氧基甲基苯丙胺、3,4-亚甲二氧基苯丙胺和 3,4-亚甲二氧基-N-乙基苯丙胺，印迹因子为 2.280∼3.698，选择性因子为 1.654∼3.698。此外，ATS 可以从水中快速吸附，平衡时间在 5 分钟内。ATS 中的氨基与 DMIP 上的羧基之间的氢键相互作用可能是主要的吸附机制。DMIP 被用作固相萃取（SPE）吸附剂。在最佳萃取条件下，基于 DMIP 的 SPE 和高效液相色谱-串联质谱法的方法在 0.025∼1.00 μmol L 的范围内表现出良好的线性，具有良好的重复性（RSD 4.8∼8.6%，n=5）和低定量下限（0.007∼0.200 ng mL，S/N=10）。对于所有目标物，包括可乐碳酸饮料、啤酒和鸡尾酒中的加标样品，均获得了令人满意的回收率（72.5∼120%）和低 RSD 值（<10%）。与其他商业 SPE 吸附剂相比，DMIP 对可乐、啤酒和鸡尾酒的基质效应（ME）较低，ME 值分别为 101∼124%、75.8∼80.2%和 103∼128%。结果表明，所开发的 DMIP 材料可能是酒精和非酒精饮料中 ATS 预处理的潜在候选材料。
