Tan Dongqin, Liang Yi, Guo Ting, Wang Yue, Li Yanying, Sun Xiaoli, Wang Degao
College of Environmental Science and Engineering, Dalian Maritime University, No. 1 Linghai Road, Dalian 116026, China.
College of Environmental Science and Engineering, Dalian Maritime University, No. 1 Linghai Road, Dalian 116026, China.
J Chromatogr A. 2023 Oct 11;1708:464368. doi: 10.1016/j.chroma.2023.464368. Epub 2023 Sep 9.
Dummy molecularly imprinted polymers (DMIPs) with high selectivity for amphetamine-type stimulants (ATSs) were synthesized using synephrine molecule as a dummy template. The polymers were irregularly massive with a specific surface area of 330 mg. Adsorption experiments found that the imprinting factors for five ATSs (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxy-N-ethylamphetamine) were 2.3∼3.7. The DMIPs-agarose gel mixed matrix membranes (MMMs) were further prepared by incorporating DMIPs in the agarose matrix. MMMs were used to extract five ATSs from wastewater and urine samples. Extraction conditions such as membrane matrix, sample pH, dissolved organic matter content, extraction time, and elution reagent were optimized. Under optimal conditions, the developed MMMs-HPLC-MS/MS method exhibited low limits of detection (0.1∼3.0ng L), satisfactory recoveries (91.7∼100%), and good repeatability (RSD<7%, n=3). It was then successfully applied to ATSs analysis in wastewater and urine samples. Recoveries of ATSs in spiked wastewater and urine were 82.0∼98.4% and 82.3∼95.7%, respectively. Moreover, compared with other methods, the present method possessed the advantages of high quantitative ability, suitable for typical environmental conditions, and low application cost. The above results suggested that the developed MMMs-HPLC-MS/MS method could be used as a feasible strategy to extract and determine trace ATSs in wastewater and urine samples.
以辛弗林分子为虚拟模板，合成了对苯丙胺类兴奋剂（ATSs）具有高选择性的虚拟分子印迹聚合物（DMIPs）。这些聚合物呈不规则块状，比表面积为330 mg。吸附实验发现，五种ATSs（苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基苯丙胺、3,4-亚甲基二氧基甲基苯丙胺和3,4-亚甲基二氧基-N-乙基苯丙胺）的印迹因子为2.3∼3.7。通过将DMIPs掺入琼脂糖基质中，进一步制备了DMIPs-琼脂糖凝胶混合基质膜（MMMs）。MMMs用于从废水和尿液样本中提取五种ATSs。对膜基质、样品pH值、溶解有机物含量、提取时间和洗脱试剂等提取条件进行了优化。在最佳条件下，所建立的MMMs-HPLC-MS/MS方法具有低检测限（0.1∼3.0ng L）、令人满意的回收率（91.7∼100%）和良好的重复性（RSD<7%，n = 3）。该方法随后成功应用于废水和尿液样本中ATSs的分析。加标废水和尿液中ATSs的回收率分别为82.0∼98.4%和82.3∼95.7%。此外，与其他方法相比，本方法具有定量能力高、适用于典型环境条件且应用成本低的优点。上述结果表明，所建立的MMMs-HPLC-MS/MS方法可作为一种可行的策略，用于提取和测定废水和尿液样本中的痕量ATSs。
