Liu Peng, Yu Xinyang, Dai Xiaohong, Zou Wei, Yu Xueping, Niu Mingming, Chen Qiuxin, Teng Wei, Kong Ying, Guan Ruiqiao, Liu Xiaoying
First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China.
Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine, Heilongjiang University of Chinese Medicine, Harbin, China.
Front Aging Neurosci. 2021 Dec 20;13:718631. doi: 10.3389/fnagi.2021.718631. eCollection 2021.
To study the effect of scalp acupuncture (SA) on the mitophagy signaling pathway in the caudate nucleus of Sprague-Dawley rats following intracerebral hemorrhage (ICH). An ICH model was established by injecting autologous arterial blood into the caudate nucleus in 200 male Sprague-Dawley rats, which were divided into five groups: sham, ICH, 3-methyladenine group (3-MA, 30 mg/kg), SA, and SA+3-MA. Animals were analyzed at 6 and 24 h as well as at 3 and 7 days. Composite neurological scale score was significantly higher in the SA group than in the ICH group. Transmission electron microscopy showed less structural damage and more autophagic vacuoles within brain in the SA group than in the ICH group. SA group showed higher levels of Beclin1, Parkin, PINK1, NIX protein, and a lower level of Caspase-9 in brain tissue. These animals consequently showed less neural cell apoptosis. Compared with the SA group, however, the neural function score and levels of mitophagy protein in the SA+3-MA group were decreased, neural cell apoptosis was increased with more severe structural damage, which suggested that 3-MA may antagonize the protective effect of SA on brain in rats with ICH. SA may mitigate the neurologic impairment after ICH by enhancing mitophagy and reducing apoptosis.
研究头皮针（SA）对脑出血（ICH）后Sprague-Dawley大鼠尾状核线粒体自噬信号通路的影响。将200只雄性Sprague-Dawley大鼠通过向尾状核注射自体动脉血建立ICH模型，分为五组：假手术组、ICH组、3-甲基腺嘌呤组（3-MA，30mg/kg）、SA组和SA+3-MA组。在6小时和24小时以及3天和7天时对动物进行分析。SA组的综合神经量表评分显著高于ICH组。透射电子显微镜显示，SA组脑内结构损伤较ICH组少，自噬空泡更多。SA组脑组织中Beclin1、Parkin、PINK1、NIX蛋白水平较高，Caspase-9水平较低。这些动物的神经细胞凋亡因此较少。然而，与SA组相比，SA+3-MA组的神经功能评分和线粒体自噬蛋白水平降低，神经细胞凋亡增加，结构损伤更严重，这表明3-MA可能拮抗SA对ICH大鼠脑的保护作用。SA可能通过增强线粒体自噬和减少细胞凋亡来减轻ICH后的神经功能损害。
