高强度间歇训练与人类羊膜支架对大鼠股四头肌体积损失损伤后胫骨前肌血管化和神经支配的联合作用。
Simultaneous Effects of High Intensity Interval Training and Human Amniotic Membrane Scaffold on Rat Tibialis Anterior Vascularization and Innervation after Volumetric Muscle Loss Injury.
作者信息
Izadi M R, Habibi A, Khodabandeh Z, Nikbakht M
机构信息
Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Stem Cell Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Int J Organ Transplant Med. 2021;12(1):33-43.
BACKGROUND
Despite the high regenerative capacity of skeletal muscle, volumetric muscle loss (VML) is an irrecoverable injury. One therapeutic approach is the implantation of engineered biologic scaffolds.
OBJECTIVE
To investigate the simultaneous effect of high intensity interval training (HIIT) and the use of decellularized human amniotic membrane (dHAM) scaffolds on vascularization, growth factor, and neurotrophic factor gene expression, and muscle force generation in the tibialis anterior (TA) of rats after VML injury.
METHODS
VML injury was created in the TA of 24 rats, which were randomly divided into two groups-12 animals with and 12 without the use of a dHAM scaffold. After injury, each group was further divided into two groups of 6 animals each-sedentary and HIIT. Blood vessels were visualized and counted by hematoxylin and eosin staining. The PowerLab converter assay was used to evaluate isometric contraction force. The relative expression of neurotrophic factors and growth factor genes was measured with reverse transcription PCR (RT-PCR).
RESULTS
The number of blood vessels in the whole regenerating areas showed a significant difference in the dHAM-HIIT and dHAM-sedentary groups compared to the sedentary group without dHAM (p=0.001 and p=0.003, respectively). and mRNA levels in the dHAM-HIIT group were significantly (p<0.05) higher than those in other groups; mRNA levels did not differ significantly among groups. Isometric contraction force in the dHAM-HIIT group was significantly (p=0.001) greater compared to the sedentary group without dHAM.
CONCLUSION
Combined use of dHAM scaffoldsand HIIT would improve the structure of the injured muscle during regeneration after VML by better vascular perfusion. HIIT leads to greater force generation and innervation by modulating neurotrophic factor synthesis in regenerating muscles.
背景
尽管骨骼肌具有较高的再生能力，但容积性肌肉损失（VML）仍是一种不可恢复的损伤。一种治疗方法是植入工程化生物支架。
目的
研究高强度间歇训练（HIIT）和使用脱细胞人羊膜（dHAM）支架对VML损伤大鼠胫前肌（TA）血管生成、生长因子和神经营养因子基因表达以及肌肉力量产生的联合作用。
方法
在24只大鼠的TA中造成VML损伤，将其随机分为两组，每组12只，一组使用dHAM支架，另一组不使用。损伤后，每组再进一步分为两组，每组6只，分别为久坐组和HIIT组。通过苏木精和伊红染色观察并计数血管。使用PowerLab转换器测定法评估等长收缩力。用逆转录PCR（RT-PCR）检测神经营养因子和生长因子基因的相对表达。
结果
与不使用dHAM的久坐组相比，dHAM-HIIT组和dHAM-久坐组整个再生区域的血管数量有显著差异（分别为p = 0.001和p = 0.003）。dHAM-HIIT组中 和 的mRNA水平显著高于其他组（p < 0.05）； 的mRNA水平在各组间无显著差异。与不使用dHAM的久坐组相比，dHAM-HIIT组的等长收缩力显著更大（p = 0.001）。
结论
联合使用dHAM支架和HIIT可通过改善血管灌注，在VML后再生过程中改善受损肌肉的结构。HIIT通过调节再生肌肉中神经营养因子的合成，导致更大的力量产生和神经支配。
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