Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, P.O. Box 11562, Egypt.
Obstetrics and Gynecology Department, Faculty of Medicine, Cairo University, Egypt.
J Adv Res. 2016 Mar;7(2):233-41. doi: 10.1016/j.jare.2015.05.002. Epub 2015 May 30.
The present study was conducted to compare between the therapeutic efficacies of human amniotic membrane-derived stem cells (hAM-MSCs) vs. adipose tissue derived stem cells (AD-MSCs) in cyclophosphamide (CTX)-induced ovarian failure in rats. Forty-eight adult female rats were included in the study; 10 rats were used as control group. Thirty-eight rats were injected with CTX to induce ovarian failure and divided into four groups: ovarian failure (IOF) (IOF group), IOF + phosphate buffer saline (PBS group), IOF + hAM-MSCs group and IOF + AD-MSCs group. Serum levels of FSH and estradiol (E2) were assessed. Histopathological examination of the ovarian tissues was performed and quantitative gene expressions of Oct-4, Stra8 and integrin beta-1 genes were conducted by quantitative real time PCR. Results showed that IOF and IOF + PBS rat groups exhibited decreased ovarian follicles, increased interstitial fibrosis with significant decrease of serum E2, significant increase serum FSH level and significant down-regulation of Stra8 and integrin beta-1. In hAM-MSCs and AD-MSCs rat groups, there were increased follicles and corpora with evident the presence of oocytes, significant increase in serum E2, significant decrease in serum FSH levels (in hAM-MSCs treated group only) and significant up-regulation of the three studied genes with higher levels in hAM-MSCs treated rats group when compared to AD-MSCs treated rats group. In Conclusion, administration of either hAM-derived MSCs or AD-MSCs exerts a significant therapeutic efficacy in chemotherapy induced ovarian insult in rats. hAM-MSCs exert higher therapeutic efficacy as compared to AD-MSCs.
本研究旨在比较人羊膜间充质干细胞（hAM-MSCs）与脂肪组织来源的间充质干细胞（AD-MSCs）在环磷酰胺（CTX）诱导的大鼠卵巢衰竭中的治疗效果。将 48 只成年雌性大鼠纳入研究，其中 10 只作为对照组。38 只大鼠注射 CTX 诱导卵巢衰竭，并分为四组：卵巢衰竭（IOF）组（IOF 组）、IOF+磷酸盐缓冲液（PBS）组、IOF+hAM-MSCs 组和 IOF+AD-MSCs 组。评估血清卵泡刺激素（FSH）和雌二醇（E2）水平。对卵巢组织进行组织病理学检查，并通过实时定量 PCR 检测 Oct-4、Stra8 和整合素β-1 基因的定量表达。结果显示，IOF 和 IOF+PBS 大鼠组卵巢卵泡减少，间质纤维化增加，血清 E2 显著降低，血清 FSH 水平显著升高，Stra8 和整合素β-1 显著下调。在 hAM-MSCs 和 AD-MSCs 大鼠组中，卵泡和黄体增多，可见卵母细胞，血清 E2 显著增加，血清 FSH 水平显著降低（仅在 hAM-MSCs 处理组中），三个研究基因的表达显著上调，且 hAM-MSCs 处理组的水平高于 AD-MSCs 处理组。结论：hAM 来源的 MSCs 或 AD-MSCs 的给药在大鼠化疗诱导的卵巢损伤中具有显著的治疗效果。hAM-MSCs 的治疗效果优于 AD-MSCs。
