McCarthy B M, Okano Y, Nakayasu T, Macy M, Watson S R, Harmony J A
Department of Pharmacology and Cell Biophysics, University of Cincinnati, OH 45267-0575.
J Lipid Res. 1987 Sep;28(9):1067-77.
Lipoproteins of hydrated densities less than 1.063 g/ml, very low density (VLDL) and low density (LDL) lipoproteins, could both enhance and suppress the proliferation of T lymphocyte cell lines. Enhancement and suppression were dependent on lipoprotein and transferrin concentrations. Enhancement occurred at low lipoprotein and high transferrin; suppression, at high lipoprotein and low transferrin. Lipoprotein suppression required a constituent of cell-conditioned medium as evidenced by the fact that lipoproteins did not suppress the replicative response of the IL-2-dependent murine cell line CTLL-2 to purified IL-2 but could suppress the response to cell-conditioned medium IL-2. For lipoprotein suppression and its relief by transferrin, both growth-regulating factors were required early in the cell cycle, suggesting that events important to progression through G1 are influenced. The data establish that the interplay between plasma lipoproteins, transferrin, and an unknown constituent of cell-conditioned medium can regulate the proliferation of T lymphocytes.
水合密度小于1.063克/毫升的脂蛋白，即极低密度（VLDL）脂蛋白和低密度（LDL）脂蛋白，既能增强也能抑制T淋巴细胞系的增殖。增强和抑制取决于脂蛋白和转铁蛋白的浓度。在低脂蛋白和高转铁蛋白浓度时发生增强作用；在高脂蛋白和低转铁蛋白浓度时发生抑制作用。脂蛋白抑制作用需要细胞条件培养基的一种成分，这一事实表明脂蛋白并不抑制依赖白细胞介素-2的小鼠细胞系CTLL-2对纯化白细胞介素-2的复制反应，但能抑制对细胞条件培养基白细胞介素-2的反应。对于脂蛋白抑制作用及其被转铁蛋白解除抑制的情况，两种生长调节因子在细胞周期早期都是必需的，这表明对通过G1期进展至关重要的事件受到了影响。这些数据表明，血浆脂蛋白、转铁蛋白和细胞条件培养基中一种未知成分之间的相互作用可以调节T淋巴细胞的增殖。
