Kim Han Woo, Kim Jiyeun Kate, Park Indal, Lee Sang Joon
Department of Microbiology, College of Medicine, Kosin University, Busan, South Korea.
Department of Ophthalmology, College of Medicine, Kosin University, Busan, South Korea.
Front Med (Lausanne). 2021 Dec 20;8:670199. doi: 10.3389/fmed.2021.670199. eCollection 2021.
To establish ocular co-culture models of and and to study how various concentrations of moxifloxacin affect the survival of these two endophthalmitis-causing bacteria. Standard strains of and were used. Color detection agar plates were employed to distinguish their colonies. To establish the and co-culture models, and were co-cultivated at different ratios for various periods. For the model, various volumes and concentrations of either a mono-culture or co-culture were inoculated into the lower conjunctival sac of rabbits. Finally, the newly developed and co-culture models were subjected to the moxifloxacin treatment to access its effect on and . When and were cultured separately in tryptic soy broth, their growth peaked and plateaued at approximately 16 and 6 h, respectively. When they were co-cultured, the growth peak of got delayed, whereas the growth peak of did not change. The number of was significantly higher in the co-culture than that in the mono-culture. Treatment with moxifloxacin in the co-culture model rapidly decreased the number of cells at doses ≥ 0.125 μg/ml. In contrast, the number of did not change significantly up to 16 μg/ml moxifloxacin. In co-culture (at 1:1), the count decreased in a pattern similar to that seen in mono-culture and was barely detectable at 24 h after inoculation. In contrast, the of count increased up to 16 h and then decreased. When moxifloxacin was applied (zero, one, or two times) to this model, the count decreased in proportion to the number of treatments. In contrast, the count increased with moxifloxacin treatment. The and co-culture models of and were established to determine the influence of moxifloxacin eye drops on these bacteria. The results clearly show that the moxifloxacin eye drops can make dominant on the ocular surface.
建立金黄色葡萄球菌和表皮葡萄球菌的眼共培养模型，并研究不同浓度的莫西沙星如何影响这两种引起眼内炎的细菌的存活。使用金黄色葡萄球菌和表皮葡萄球菌的标准菌株。采用显色检测琼脂平板来区分它们的菌落。为建立金黄色葡萄球菌和表皮葡萄球菌共培养模型，将金黄色葡萄球菌和表皮葡萄球菌以不同比例共培养不同时间。对于金黄色葡萄球菌模型，将不同体积和浓度的单培养物或共培养物接种到兔的下结膜囊中。最后，对新建立的金黄色葡萄球菌和表皮葡萄球菌共培养模型进行莫西沙星处理，以评估其对金黄色葡萄球菌和表皮葡萄球菌的作用。当金黄色葡萄球菌和表皮葡萄球菌分别在胰蛋白胨大豆肉汤中培养时，它们的生长峰值分别在约16小时和6小时达到并趋于平稳。当它们共培养时，金黄色葡萄球菌的生长峰值延迟，而表皮葡萄球菌的生长峰值没有变化。共培养中金黄色葡萄球菌的数量明显高于单培养。在金黄色葡萄球菌共培养模型中，莫西沙星处理剂量≥0.125μg/ml时可迅速减少金黄色葡萄球菌细胞数量。相比之下，在莫西沙星浓度高达16μg/ml时，表皮葡萄球菌数量没有明显变化。在金黄色葡萄球菌与表皮葡萄球菌1:1共培养中，金黄色葡萄球菌数量的下降模式与单培养相似，接种后24小时几乎检测不到。相比之下，表皮葡萄球菌数量在16小时前增加，然后下降。当对该模型应用莫西沙星（零次、一次或两次）时，金黄色葡萄球菌数量与处理次数成比例下降。相比之下，表皮葡萄球菌数量随莫西沙星处理而增加。建立了金黄色葡萄球菌和表皮葡萄球菌的共培养模型，以确定莫西沙星滴眼液对这些细菌的影响。结果清楚地表明，莫西沙星滴眼液可使金黄色葡萄球菌在眼表占优势。
