Mountcastle Sophie E, Cox Sophie C, Sammons Rachel L, Jabbari Sara, Shelton Richard M, Kuehne Sarah A
EPSRC Centre for Doctoral Training in Physical Sciences for Health, University of Birmingham, Birmingham, UK.
School of Dentistry, University of Birmingham, Birmingham, UK.
J Oral Microbiol. 2020 Jun 4;12(1):1773122. doi: 10.1080/20002297.2020.1773122.
Co-cultures allow for the study of cell-cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these systems have developed significantly from simple 2D eukaryotic cultures and planktonic bacteria to more complex 3D tissue engineered structures and biofilms. Careful selection and design of the co-culture along with critical parameters, such as seeding density and choice of analysis method, have resulted in several advances. This review provides a comparison of existing co-culture systems for the oral environment, with emphasis on progression of 3D models and the opportunity to harness techniques from other fields to improve current methods. While filling a gap in navigating this literature, this review ultimately supports the development of this vital technique in the field of oral biology.
共培养有助于研究不同真核生物物种之间或与细菌的细胞间相互作用。这种方法使研究人员能够更紧密地模拟复杂的组织结构。本综述聚焦于模拟口腔的共培养系统，这些系统已被用于评估这种独特的细胞环境并了解疾病进展。随着时间的推移，这些系统已从简单的二维真核生物培养和浮游细菌显著发展为更复杂的三维组织工程结构和生物膜。对共培养以及关键参数（如接种密度和分析方法的选择）进行仔细的选择和设计已带来了多项进展。本综述对现有的口腔环境共培养系统进行了比较，重点关注三维模型的进展以及利用其他领域技术改进现有方法的机会。在填补浏览该文献的空白的同时，本综述最终支持了这一口腔生物学领域重要技术的发展。
