Fujian Key Laboratory of Functional Marine Sensing Materials, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, P.R. China.
MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, P.R. China.
Angew Chem Int Ed Engl. 2022 Mar 7;61(11):e202113795. doi: 10.1002/anie.202113795. Epub 2022 Jan 24.
Systematic interrogation of correlative signaling components in their native environment is of great interest for dissecting sophisticated cellular signaling. However, it remains a challenge because of the lack of versatile and effective approaches. Herein, we propose a cell membrane-anchored DNA multitasking processor acting as a "traffic light" for integrated analyses of cellular signal transduction. Enhanced and controllable inhibition of c-Met signaling was achieved by membrane-anchoring of DNA processors. Moreover, the multitasking capability of the DNA processor allowed the monitoring of correlative VEGF secretion induced by c-Met activity regulation directly. By exploiting versatile aptameric nucleic acids, this modular designed DNA multitasking processor dissected how cell surface receptors coordinated with related components in live cells systematically. Therefore, it provides a powerful chemical tool for both fundamental cell biology research and precision medicine applications.
系统地研究相关信号成分在其天然环境中的作用对于解析复杂的细胞信号转导非常重要。然而，由于缺乏通用且有效的方法，这仍然是一个挑战。在此，我们提出了一种细胞膜锚定的 DNA 多功能处理器，它可以作为“交通灯”，用于整合分析细胞信号转导。通过将 DNA 处理器锚定在细胞膜上，可以增强和控制 c-Met 信号的抑制。此外，DNA 处理器的多功能性允许直接监测由 c-Met 活性调节诱导的相关 VEGF 分泌。通过利用多功能适体核酸，这种模块化设计的 DNA 多功能处理器在活细胞中系统地解析了细胞表面受体如何与相关成分协同作用。因此，它为基础细胞生物学研究和精准医学应用提供了一种强大的化学工具。
