Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Q Rev Biophys. 2022 May 16;55:e5. doi: 10.1017/S003358352200004X.
Lipid-DNA conjugates have emerged as highly useful tools to modify the cell membranes. These conjugates generally consist of a lipid anchor for membrane modification and a functional DNA nanostructure for membrane analysis or regulation. There are several unique properties of these lipid-DNA conjugates, especially including their programmability, fast and efficient membrane insertion, and precise sequence-specific assembly. These unique properties have enabled a broad range of biophysical applications on live cell membranes. In this review, we will mainly focus on recent tremendous progress, especially during the past three years, in regulating the biophysical features of these lipid-DNA conjugates and their key applications in studying cell membrane biophysics. Some insights into the current challenges and future directions of this interdisciplinary field have also been provided.
脂质-DNA 缀合物已成为修饰细胞膜的非常有用的工具。这些缀合物通常由用于膜修饰的脂质锚和用于膜分析或调节的功能性 DNA 纳米结构组成。这些脂质-DNA 缀合物具有几个独特的性质，特别是包括它们的可编程性、快速高效的膜插入和精确的序列特异性组装。这些独特的性质使得它们在活细胞膜的广泛生物物理应用成为可能。在这篇综述中，我们将主要关注过去三年中，在调节这些脂质-DNA 缀合物的生物物理特性及其在研究细胞膜生物物理学中的关键应用方面取得的巨大进展。还提供了对这个跨学科领域当前挑战和未来方向的一些见解。
