Hopf U, Meyer zum Büschenfelde K H
Z Gastroenterol. 1978 Apr;16(4):261-4.
A simplified method for the detection of liver membrane autoantibodies (LMA) in patient's serum is presented. Hepatocytes are isolated from the liver of young rabbits. A calcium-free solution without enzymes is injected into the portal vein with a normal syringe. The liver is then cut into small pieces in a solution with calcium, shaken in a water bath, filtered and washed in Eagle's medium. The isolated hepatocytes are incubated first in patient's serum and secondly in FITC labeled antihuman IgG. The test requires three hours and allow the detection of LMA, antinuclear and antimitochondrial antibodies simultaneously. The detection of LMA is valuable in the diagnosis of autoimmune chronic active hepatitis.
本文介绍了一种检测患者血清中肝细胞膜自身抗体（LMA）的简化方法。从幼兔肝脏中分离出肝细胞。用普通注射器将不含酶的无钙溶液注入门静脉。然后将肝脏在含钙溶液中切成小块，在水浴中振荡，过滤并在伊格尔培养基中洗涤。分离出的肝细胞先在患者血清中孵育，然后在异硫氰酸荧光素标记的抗人IgG中孵育。该检测需要三个小时，可同时检测LMA、抗核抗体和抗线粒体抗体。LMA的检测对自身免疫性慢性活动性肝炎的诊断具有重要价值。
