Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden.
Biochim Biophys Acta Biomembr. 2022 Apr 1;1864(4):183857. doi: 10.1016/j.bbamem.2021.183857. Epub 2022 Jan 4.
The plasma membrane (PM) is a highly heterogenous structure intertwined with the cortical actin cytoskeleton and extracellular matrix. This complex architecture makes it difficult to study the processes taking place at the PM. Model membrane systems that are simple mimics of the PM overcome this bottleneck and allow us to study the biophysical principles underlying the processes at the PM. Among them, cell-derived giant plasma membrane vesicles (GPMVs) are considered the most physiologically relevant system, retaining the compositional complexity of the PM to a large extent. GPMVs have become a key tool in membrane research in the last few years. In this review, I will provide a brief overview of this system, summarize recent applications and discuss the limitations.
质膜（PM）是一种高度异质的结构，与皮质肌动蛋白细胞骨架和细胞外基质交织在一起。这种复杂的结构使得研究质膜上发生的过程变得困难。模型膜系统是 PM 的简单模拟，可以克服这个瓶颈，使我们能够研究质膜上发生的过程的生物物理原理。其中，细胞衍生的巨大质膜囊泡（GPMV）被认为是最具生理相关性的系统，在很大程度上保留了 PM 的组成复杂性。GPMV 近年来已成为膜研究的重要工具。在这篇综述中，我将简要概述这个系统，总结最近的应用，并讨论其局限性。
