Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Food Chem. 2022 May 30;377:131942. doi: 10.1016/j.foodchem.2021.131942. Epub 2021 Dec 31.
Fabricated pea protein isolate (PPI) nanofibrils were used as nanocarriers to encapsulate, stabilize and deliver resveratrol (RES). PPI nanofibrils possessed a dramatically higher surface hydrophobicity than PPI (native), and PPI nanofibrils exhibited nanoscale widths of 10 nm and average lengths of 1.0 μm. Fluorescence analyses demonstrated PPI nanofibrils had high binding constant with RES. Compared with RES (free), the aqueous solubility of RES was improved by approximately 1000-fold with PPI nanofibrils complex. DPPH and ABTS radical scavenging activity assays showed that the antioxidant capacity of RES was pronouncedly enhanced through the nanocomplexation with PPI nanofibrils. RES-PPI nanofibrils complexes exhibited higher antiproliferative activities than RES (free), with the cell viabilities of 52.6% and 38.5% for RES (free) and RES-PPI nanofibrils complex at 20 μg/mL. This study demonstrates that PPI nanofibrils can be utilized as novel nanocarriers for improvements of the water solubility, chemical stability and in vitro biological activities of hydrophobic nutraceuticals.
我们使用人工豌豆蛋白分离物（PPI）纳米纤维作为纳米载体来包封、稳定和输送白藜芦醇（RES）。PPI 纳米纤维的表面疏水性明显高于 PPI（天然），PPI 纳米纤维的宽度为 10nm，平均长度为 1.0μm。荧光分析表明 PPI 纳米纤维与 RES 具有高结合常数。与 RES（游离态）相比，RES 与 PPI 纳米纤维复合后，其水溶解度提高了约 1000 倍。DPPH 和 ABTS 自由基清除活性测定表明，通过与 PPI 纳米纤维的纳米复合，RES 的抗氧化能力显著增强。RES-PPI 纳米纤维复合物的增殖活性低于 RES（游离态），在 20μg/mL 时，RES（游离态）和 RES-PPI 纳米纤维复合物的细胞活力分别为 52.6%和 38.5%。本研究表明，PPI 纳米纤维可用作新型纳米载体，提高疏水性营养药物的水溶性、化学稳定性和体外生物活性。
