Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, China.
Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, China.
Food Res Int. 2022 Oct;160:111713. doi: 10.1016/j.foodres.2022.111713. Epub 2022 Jul 21.
The current research aims to construct and assess pea protein isolate (PPI) nanocarriers for lipophilic polyphenols of curcumin (CUR), quercetin (QUE) and resveratrol (RES), respectively. Fluorescence analysis demonstrated that the binding affinity declined in sequence of QUE > CUR > RES and about one polyphenol compound was bound to protein. Thermodynamic parameters revealed that hydrophobic interaction was mainly responsible for complexation between CUR/RES and PPI, while hydrogen bonding for QUE with PPI. All nanoparticles showed particle size of 154-159 nm. Three lipophilic polyphenols were successfully encapsulated into PPI, with loading capacity of RES > QUE > CUR. Complexation of three polyphenols did not change the secondary structure of PPI. Results of FTIR, DSC and XRD confirmed that polyphenols changed from crystalline to amorphous state after combination with PPI. SEM pictures exhibited regular spherical microstructure of nanocomplexes. PPI shielded polyphenols from sensitive environment of ultraviolet light and thermal treatment. ABTS and DPPH radical scavenging activity of polyphenols were considerably improved through complexation with PPI. Molecular docking studies showed binding energy with 11S legumin in sequence of QUE > RES > CUR, and stronger hydrogen bonds were built between QUE and the protein than the other two polyphenols. Data in the present work may provide helpful information for encapsulation of lipophilic polyphenols with pea protein and the potential application in food science, pharmaceutical and cosmetics industries in the future.
本研究旨在构建并评估豌豆分离蛋白（PPI）纳米载体，分别用于包载脂溶性多酚类化合物姜黄素（CUR）、槲皮素（QUE）和白藜芦醇（RES）。荧光分析表明，结合亲和力按 QUE > CUR > RES 的顺序递减，约有一个多酚化合物与蛋白质结合。热力学参数表明，CUR/RES 与 PPI 之间的复合主要是由疏水相互作用引起的，而 QUE 与 PPI 之间则是由氢键引起的。所有纳米颗粒的粒径均为 154-159nm。三种脂溶性多酚类化合物均成功包载于 PPI 中，载药量为 RES > QUE > CUR。三种多酚类化合物的复合并未改变 PPI 的二级结构。傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）和 X 射线衍射（XRD）的结果证实，多酚类化合物与 PPI 结合后由晶态转变为非晶态。扫描电子显微镜（SEM）照片显示纳米复合物具有规则的球形微观结构。PPI 可保护多酚类化合物免受紫外光和热处理等敏感环境的影响。通过与 PPI 复合，多酚类化合物的 ABTS 和 DPPH 自由基清除活性得到显著提高。分子对接研究表明，与 11S 球蛋白的结合能按 QUE > RES > CUR 的顺序递减，QUE 与蛋白质之间形成的氢键比其他两种多酚类化合物更强。本研究结果可为利用豌豆蛋白包埋脂溶性多酚类化合物提供有价值的信息，并有望在未来的食品科学、制药和化妆品行业得到应用。
