Abe Y, Sagawa T, Sakai K, Kimura S
Second Department of Surgery, Ehime University, School of Medicine, Japan.
Clin Chim Acta. 1987 Sep 15;168(1):87-95. doi: 10.1016/0009-8981(87)90270-1.
An enzyme-linked immunosorbent assay for human epidermal growth factor (hEGF) by the sandwich method with the use of orthophenylene diamine (OPDA) for the substrate of the enzyme reaction was developed. This assay showed high sensitivity, and there was no problem with the recovery rates even without any pretreatment of samples. The changes of hEGF levels in plasma and serum depend on the condition of blood storage from sampling until separation of the cell content. The levels of hEGF markedly increased with time in serum, especially when stored at room temperature but less so in plasma. Changes of hEGF levels in plasma were negligible for up to 3 h if the blood was stored at 4 degrees C until separation.
开发了一种用于人表皮生长因子（hEGF）的酶联免疫吸附测定法，采用夹心法，使用邻苯二胺（OPDA）作为酶反应的底物。该测定法显示出高灵敏度，即使样品未经任何预处理，回收率也没有问题。血浆和血清中hEGF水平的变化取决于从采血到细胞内容物分离期间的血液储存条件。血清中hEGF水平随时间显著增加，尤其是在室温下储存时，但在血浆中增加较少。如果血液在4℃下储存直至分离，血浆中hEGF水平在长达3小时内的变化可以忽略不计。
