Guo Lili, Zhao Yongda, Huan Yanjun
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
National Risk Assessment Laboratory for Antimicrobial Resistance of Microorganisms in Animals, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Front Cell Dev Biol. 2021 Dec 21;9:803181. doi: 10.3389/fcell.2021.803181. eCollection 2021.
Chlorpyrifos (CPF), a widely used organophosphate pesticide, is reported to severely impair mammalian reproductive system. Pterostilbene (PTS), an effective free radical scavenger, is considered as beneficial for mammalian reproduction. However, the toxicity of CPF on oocyte maturation and whether PTS can eliminate the detrimental effect of CPF on oocytes remain unclear. Here, porcine oocytes were applied to investigate the potential effect and possible mechanism of CPF and PTS during oocyte maturation. This work demonstrated that CPF significantly delayed the meiotic progression and decreased the polar body extrusion by disturbing spindle assembly and chromosome alignment and causing DNA damage in oocytes ( < 0.05). And, CPF significantly impaired oocyte cytoplasmic maturation by inducing the high level of reactive oxygen species and decreasing glutathione content ( < 0.05). Moreover, CPF significantly triggered embryo apoptosis and reduced the blastocyst rate and cell number following parthenogenetic activation ( < 0.05). Whereas CPF-exposed oocytes were treated with PTS, these defects caused by CPF were obviously rescued, and oocyte maturation and subsequent embryonic development were also significantly ameliorated ( < 0.05). In conclusion, these results revealed that CPF exerted the toxic effect on porcine oocytes, while PTS effectively alleviated CPF-induced damage on oocytes. This work provides a potential strategy to protect oocyte maturation in mammalian species.
毒死蜱（CPF）是一种广泛使用的有机磷农药，据报道会严重损害哺乳动物的生殖系统。紫檀芪（PTS）是一种有效的自由基清除剂，被认为对哺乳动物生殖有益。然而，CPF对卵母细胞成熟的毒性以及PTS是否能消除CPF对卵母细胞的有害影响仍不清楚。在此，应用猪卵母细胞来研究CPF和PTS在卵母细胞成熟过程中的潜在作用及可能机制。这项研究表明，CPF通过干扰纺锤体组装和染色体排列并导致卵母细胞DNA损伤，显著延迟减数分裂进程并降低极体排出率（P<0.05）。并且，CPF通过诱导高水平的活性氧并降低谷胱甘肽含量，显著损害卵母细胞的细胞质成熟（P<0.05）。此外，CPF显著引发胚胎凋亡，并降低孤雌激活后的囊胚率和细胞数量（P<0.05）。而用PTS处理暴露于CPF的卵母细胞后，CPF引起的这些缺陷明显得到挽救，卵母细胞成熟及随后的胚胎发育也得到显著改善（P<0.05）。总之，这些结果表明CPF对猪卵母细胞产生毒性作用，而PTS有效减轻了CPF对卵母细胞的损伤。这项研究为保护哺乳动物物种的卵母细胞成熟提供了一种潜在策略。
