Hirayama Y, Inaba K, Komatsubara S, Yoshida K, Kawai J, Naito K, Muramatsu S
Department of Zoology, Faculty of Science, Kyoto University, Japan.
Immunology. 1987 Nov;62(3):393-9.
Accessory cell (A cell) functions of splenic dendritic cells (DC) and peritoneal macrophages (M phi) were investigated in the Con A-stimulated proliferative response of thymic T cells. DC were more efficient as A cells than M phi in respect of their necessary cell numbers, Con A dose and culture period required for optimal response. Con A-pulsed T cells proliferated with the aid of lymphocyte activating factor(s) (LAF) derived from M phi, even in the apparent absence of A cells. Con A-pulsed M phi were superior to unpulsed M phi in the secretion of LAF to induce a high response of Con A-pulsed T cells. A cell activity of M phi in different preparations appeared to parallel the ability to secrete LAF, and was totally abolished by fixation of M phi with paraformaldehyde. The fixation of DC, however, resulted in only a partial reduction of the A cell activity. These results argue that both DC and M phi can serve as A cells in the T-cell response to Con A, but that the mechanism to manifest A cell activity is somewhat different between DC and M phi.
在伴刀豆球蛋白A（Con A）刺激的胸腺T细胞增殖反应中，研究了脾树突状细胞（DC）和腹腔巨噬细胞（M phi）的辅助细胞（A细胞）功能。就最佳反应所需的必要细胞数量、Con A剂量和培养期而言，DC作为A细胞比M phi更有效。即使在明显没有A细胞的情况下，Con A刺激的T细胞也能借助源自M phi的淋巴细胞激活因子（LAF）进行增殖。Con A刺激的M phi在分泌LAF以诱导Con A刺激的T细胞产生高反应方面优于未刺激的M phi。不同制剂中M phi的A细胞活性似乎与分泌LAF的能力平行，并且用多聚甲醛固定M phi可使其完全丧失活性。然而，DC的固定仅导致A细胞活性部分降低。这些结果表明，DC和M phi在T细胞对Con A的反应中均可作为A细胞，但DC和M phi表现出A细胞活性的机制有所不同。
