Carroll A M, Greene M I
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia.
Immunology. 1987 Nov;62(3):471-5.
Injection of the hapten azobenzenearsonate (ABA) under mouse ear skin results in increased weight and accumulation of injected radiolabelled syngeneic lymphocytes in the draining auricular lymph node relative to the contralateral node. Intravenous injection of ABA hapten-coupled spleen cells results in a similar accumulation of labelled cells in recipient spleens. The kinetics, antigen-dose effects and enhancement by priming indicate an active host-initiated lymphocyte trapping response. ABA-induced splenic and nodal trapping and increased lymph node weight were prevented by in vivo administration of anti-I-A monoclonal antibody prior to and coincident with antigen injection. Anti-I-A antibody did not significantly alter traffic of labelled T-enriched lymphocytes in non-antigen draining tissues.
在小鼠耳下皮肤注射半抗原偶氮苯砷酸盐（ABA）后，相对于对侧淋巴结，引流耳前淋巴结中注射的放射性标记同基因淋巴细胞的重量增加且出现积聚。静脉注射ABA半抗原偶联的脾细胞会使标记细胞在受体脾脏中出现类似的积聚。其动力学、抗原剂量效应以及预激发增强效应表明这是一种由宿主主动引发的淋巴细胞捕获反应。在注射抗原之前及同时进行体内抗I-A单克隆抗体给药，可防止ABA诱导的脾脏和淋巴结捕获以及淋巴结重量增加。抗I-A抗体不会显著改变非抗原引流组织中标记的富含T淋巴细胞的迁移。
