School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
Food Funct. 2022 Jan 24;13(2):880-890. doi: 10.1039/d1fo02282b.
Polyphenols exhibit potential functional activities, especially rosmarinic acid (RosA) and caffeic acid (CafA). In this study, two different methods, ultrasonic-assisted ethanol extraction (60%) and ultrasound-assisted cellulase (≥15 000 Ug, 2%) hydrolysis, were used for the extraction of the total phenolics from 44 species of . The Folin-Ciocalteu method of detection showed that the content of the total phenolics extracted by cellulase hydrolysis was the highest and attained up to 28.00 mg per g for ZB1. Continuously, the extracts were purified using XDA-8 macroporous resin and medium-pressure liquid chromatography (MPLC), and the content of the total phenolics improved to 66.62 mg per g. A high-performance liquid chromatography (HPLC) assay showed that the total polyphenols were mainly composed of gallic acid, caffeic acid, rosmarinic acid, luteolin and apigenin. Besides, a sequential XDA-8 macroporous resin combined with high-speed counter-current chromatography (HSCCC)/MPLC system was established for the simultaneous isolation and preparation of RosA (purity 98.29%) and CafA (purity 97.01%) from the extracts. Furthermore, the antibacterial activities of the total polyphenols were evaluated by the disc diffusion method and scanning electron microscopy (SEM) observation. The results verified that the total polyphenols had effective antibacterial activity on three kinds of bacteria including , , and in a concentration-dependent manner. All of these results demonstrated that the ultrasound-assisted cellulase hydrolysis extraction of the total polyphenols and the proposed three-step separation of RosA and CafA gave high yields and good purity, and they exhibited effective antibacterial ability.
多酚表现出潜在的功能活性，特别是迷迭香酸（RosA）和咖啡酸（CafA）。在这项研究中，使用了两种不同的方法，超声辅助乙醇提取（60%）和超声辅助纤维素酶（≥15000Ug，2%）水解，从 44 种 中提取总酚。福林- 肖卡尔特法检测表明，纤维素酶水解提取的总酚含量最高，达到 ZB1 每克 28.00 毫克。接着，使用 XDA-8 大孔树脂和中压液相色谱（MPLC）对提取物进行纯化，总酚含量提高到 66.62 毫克/克。高效液相色谱（HPLC）分析表明，总多酚主要由没食子酸、咖啡酸、迷迭香酸、木犀草素和芹菜素组成。此外，建立了一种顺序 XDA-8 大孔树脂与高速逆流色谱（HSCCC）/MPLC 系统，用于从提取物中同时分离和制备 RosA（纯度 98.29%）和 CafA（纯度 97.01%）。此外，采用纸片扩散法和扫描电子显微镜（SEM）观察评价了总多酚的抑菌活性。结果表明，总多酚对 、 、 三种细菌具有有效的抑菌活性，且呈浓度依赖性。所有这些结果表明，超声辅助纤维素酶水解提取总多酚和提出的三步分离 RosA 和 CafA 具有较高的产率和良好的纯度，并表现出有效的抑菌能力。
