Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Qinhuangdao 066000, China.
Molecules. 2023 Oct 9;28(19):6990. doi: 10.3390/molecules28196990.
is a highly pathogenic microorganism that can cause human bacillary dysentery by contaminating food and drinking water. This study investigated the antibacterial activity of chestnut bur polyphenol extract (CBPE) on and the underlying mechanism. The results showed that the minimum inhibitory concentration (MIC) of CBPE for was 0.4 mg/mL, and the minimum bactericidal concentration (MBC) was 1.6 mg/mL. CBPE treatment irreversibly disrupted cell morphology, decreased cell activity, and increased cell membrane permeability, cell membrane depolarization, and cell content leakage of , indicating that CBPE has obvious destructive effects on the cell membrane and cell wall of . Combined transcriptomic and metabolomics analysis revealed that CBPE inhibits by interfering with ABC protein transport, sulfur metabolism, purine metabolism, amino acid metabolism, glycerophospholipid metabolism, and some other pathways. These findings provide a theoretical basis for the prevention and treatment of infection with extract from chestnut burs.
志贺氏菌是一种高致病性微生物，可通过污染食物和饮用水引起人类细菌性痢疾。本研究探讨了栗蓬多酚提取物（CBPE）对志贺氏菌的抗菌活性及其作用机制。结果表明，CBPE 对志贺氏菌的最小抑菌浓度（MIC）为 0.4mg/mL，最小杀菌浓度（MBC）为 1.6mg/mL。CBPE 处理可使细胞形态不可逆转地破坏，细胞活性降低，细胞膜通透性、细胞膜去极化和细胞内容物泄漏增加，表明 CBPE 对志贺氏菌的细胞膜和细胞壁有明显的破坏作用。转录组学和代谢组学联合分析表明，CBPE 通过干扰 ABC 蛋白转运、硫代谢、嘌呤代谢、氨基酸代谢、甘油磷脂代谢和其他一些途径来抑制志贺氏菌。这些发现为栗蓬提取物预防和治疗志贺氏菌感染提供了理论依据。
